Effects of different equilibration times on post-thaw cryopreserved semen quality of cattle and buffalo bulls

Adnan Khan; R.U Khan; Muhammad Subhan Qureshi; Muhammad Mobashar; Ali Gohar; Shakoor Ahmad; Kamran  Khan; Hamayun Khan; Shabana Naz

doi:10.17582/journal.pjz/20190824040816

Effects of different equilibration times on post-thaw cryopreserved semen quality of cattle and buffalo bulls

Adnan Khan, R.U Khan, Muhammad Subhan Qureshi, Muhammad Mobashar, Ali Gohar, Shakoor Ahmad, Kamran Khan, Hamayun Khan, Shabana Naz

Agricultural, Environmental and Veterinary Sciences

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Abstract

Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality. Equilibration time of 2 and 6 hours showed detrimental effect on the semen quality.

Original language	English
Pages (from-to)	613-618
Number of pages	6
Journal	Pakistan Journal of Zoology
Volume	53
Issue number	2
Early online date	21 Feb 2021
DOIs	https://doi.org/10.17582/journal.pjz/20190824040816
Publication status	Published - Apr 2021

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title = "Effects of different equilibration times on post-thaw cryopreserved semen quality of cattle and buffalo bulls",

abstract = "Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality. Equilibration time of 2 and 6 hours showed detrimental effect on the semen quality.",

keywords = "Holstein friesian, Jersey, Buffalo breed, Nili Ravi, Semen quality",

author = "Adnan Khan and R.U Khan and Qureshi, {Muhammad Subhan} and Muhammad Mobashar and Ali Gohar and Shakoor Ahmad and Kamran Khan and Hamayun Khan and Shabana Naz",

year = "2021",

month = apr,

doi = "10.17582/journal.pjz/20190824040816",

language = "English",

volume = "53",

pages = "613--618",

journal = "Pakistan Journal of Zoology",

issn = "0030-9923",

publisher = "University of Punjab (new Campus)",

number = "2",

}

TY - JOUR

T1 - Effects of different equilibration times on post-thaw cryopreserved semen quality of cattle and buffalo bulls

AU - Khan, Adnan

AU - Khan, R.U

AU - Qureshi, Muhammad Subhan

AU - Mobashar, Muhammad

AU - Gohar, Ali

AU - Ahmad, Shakoor

AU - Khan, Kamran

AU - Khan, Hamayun

AU - Naz, Shabana

PY - 2021/4

Y1 - 2021/4

N2 - Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality. Equilibration time of 2 and 6 hours showed detrimental effect on the semen quality.

AB - Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality. Equilibration time of 2 and 6 hours showed detrimental effect on the semen quality.

KW - Holstein friesian

KW - Jersey

KW - Buffalo breed

KW - Nili Ravi

KW - Semen quality

U2 - 10.17582/journal.pjz/20190824040816

DO - 10.17582/journal.pjz/20190824040816

M3 - Article

SN - 0030-9923

VL - 53

SP - 613

EP - 618

JO - Pakistan Journal of Zoology

JF - Pakistan Journal of Zoology

IS - 2

ER -

Effects of different equilibration times on post-thaw cryopreserved semen quality of cattle and buffalo bulls

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