Effects of different equilibration times on post-thaw cryopreserved semen quality of cattle and buffalo bulls

Adnan Khan, R.U Khan, Muhammad Subhan Qureshi, Muhammad Mobashar, Ali Gohar, Shakoor Ahmad, Kamran Khan, Hamayun Khan, Shabana Naz

Research output: Contribution to journalArticlepeer-review

Abstract

Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality. Equilibration time of 2 and 6 hours showed detrimental effect on the semen quality.
Original languageEnglish
Pages (from-to)613-618
Number of pages6
JournalPakistan Journal of Zoology
Volume53
Issue number2
Early online date21 Feb 2021
DOIs
Publication statusPublished - Apr 2021

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