Effects of survivinG interference RNA on non-small cell lung carcinoma

G. F. Liu, Q. G. Zhao, L. Si, Y. G. Cao, G. Y. Li, Lexin Wang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: The primary purpose of this study was to investigate the in vitro and in vivo effect of survivin interference RNA (siRNA) on non-small cell lung cancer. Methods: Lentivirus was used as a vector to transfer siRNA into human lung cancer A549 cells. The proliferation of the cancer cells was assessed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The lentivirus-mediated siRNA was also injected into the transplanted A549 tumor tissues in mice. Tumour growth was assessed after 11 injections over a period of 21 days. Results: Compared with the placebo and the blank lentiviral vector groups, the siRNA treatment group had reduced cell growth rate following 4 days of the treatment (P<0.01). The average size of the transplanted A549 tumours in the siRNA treatment group (0.75±0.16 cm3, n=8) was smaller than in the placebo (2.09±0.22 cm3, n=6) or the blank lentivrial vector groups (1.89±0.18 cm3, n=6) (P<0.01). The tumour growth inhibition rate in the siRNA groups was 46.1%. Conclusion: Lentivirus-mediated siRNA therapy inhibits the growth of human lung cancer cells in vitro. The siRNA therapy also suppresses the growth of the transplanted lung cancer in mice.
Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalClinical and Investigative Medicine
Volume32
Issue number6
Publication statusPublished - 2009

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    Liu, G. F., Zhao, Q. G., Si, L., Cao, Y. G., Li, G. Y., & Wang, L. (2009). Effects of survivinG interference RNA on non-small cell lung carcinoma. Clinical and Investigative Medicine, 32(6), 225-231.