Activities per year
The mycotoxin lolitrem B is found in endopyte-infested perennial ryegrass (L. perenne) present in around 90% of perennial ryegrass pastures in Australia and New Zealand. Ingestion causes a neurological syndrome in grazing livestock called Perennial RyeGrass Toxicosis (PRGT) (Cunningham, 1959; Combs et al., 2014) where clinical signs include hyperexcitability, muscle tremors, ataxia (“staggers”) and, in severe cases, clonic seizures and death (Combs et al., 2014). These neurological signs are thought to result from blockade of large conductance potassium channels (BK channels) by indole diterpenoid toxins, and primarily lolitrem B (Munday-Finch SC., 1997; Imlach, 2011). Despite much investigation, the relationship between systemic levels of lolitrem B and the presence or absence of its pathway intermediaries has not been well defined.To address this question, members of the lolitrem B metabolic pathway were isolated from infested perennial ryegrass, including lolitrem B, lolitrem E and two terpendole toxins B and E, and their effects of movement quantified. Metabolomic profiling of body tissues and fluids was undertaken using HPLC-MS/MS and Quantitative Time-of-Flight analysis (LCMS/MS-QToF). Animals exposed to pure isolated lolitrem B toxin showed a significant increase in tremor and ability to maintain stability during accelerated rotarod testing, other pathway members of the family showed little or no effect. Better characterization of clinical signs and metabolic changes associated with exposure to the family of indole diterpenoid toxins present in perennial ryegrass will provide useful information for generation of grass varieties with variations in indole diterpenoid toxin production.
|Number of pages||1|
|Publication status||Published - 2016|
|Event||36th Annual Meeting of the Australasian Neuroscience Society: ANS 2016 - Hotel Grand Chancellor Hobart, Hobart, Australia|
Duration: 04 Dec 2016 → 07 Dec 2016
http://aomevents.com/ANS2016 (Conference website)
https://www.ans.org.au/resources/past-ans-conferences/57-past-ans-conferences (Link to conference abstracts and posters)
|Conference||36th Annual Meeting of the Australasian Neuroscience Society|
|Period||04/12/16 → 07/12/16|
FingerprintDive into the research topics of 'Effects of the indole diterpenoid mycotoxin lolitrem B and its pathway intermediates on movement and metabolic status in a mouse model of perennial ryegrass toxicosis'. Together they form a unique fingerprint.
- 1 Internal HDR Supervision
Clinical and Pathological Characterisation of Perennial Ryegrass Toxicosis and Investigation of Bromide as a Therapeutic Agent.01 Feb 2010 → 31 Aug 2020
Activity: Supervision/Examination/Mentoring › Internal HDR Supervision
Impact: Economic Impact
Impact: Economic ImpactFile