Electrochemical biosensing strategies for DNA methylation analysis

Tanvir Hossain, Golam Mahmudunnabi, Mostafa Kamal Masud, Md Nazmul Islam, Lezanne Ooi, Konstantin Konstantinov, Md Shahriar Al Hossain, Boris Martinac, Gursel Alici, Nam Trung Nguyen, Muhammad J.A. Shiddiky

Research output: Contribution to journalReview articlepeer-review

56 Citations (Scopus)


DNA methylation is one of the key epigenetic modifications of DNA that results from the enzymatic addition of a methyl group at the fifth carbon of the cytosine base. It plays a crucial role in cellular development, genomic stability and gene expression. Aberrant DNA methylation is responsible for the pathogenesis of many diseases including cancers. Over the past several decades, many methodologies have been developed to detect DNA methylation. These methodologies range from classical molecular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectroscopy to the more recent electrochemical approaches. Among these, electrochemical approaches offer sensitive, simple, specific, rapid, and cost-effective analysis of DNA methylation. Additionally, electrochemical methods are highly amenable to miniaturization and possess the potential to be multiplexed. In recent years, several reviews have provided information on the detection strategies of DNA methylation. However, to date, there is no comprehensive evaluation of electrochemical DNA methylation detection strategies. Herein, we address the recent developments of electrochemical DNA methylation detection approaches. Furthermore, we highlight the major technical and biological challenges involved in these strategies and provide suggestions for the future direction of this important field.
Original languageEnglish
Pages (from-to)63-73
Number of pages11
JournalBiosensors and Bioelectronics
Early online date17 Feb 2017
Publication statusPublished - 15 Aug 2017


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