TY - JOUR
T1 - Electron transport, light energy conversion and proteomic responses of periphyton in photosynthesis under exposure to AgNPs
AU - Liu, Junzhuo
AU - Zhang, Huijie
AU - Yan, Liying
AU - Kerr, Philip G.
AU - Zhang, Songhe
AU - Wu, Yonghong
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 41977101 , 41825021 and 41701301 ), the Natural Science Foundation of Jiangsu Province, China ( BK20181511 ), Key Technologies Research and Demonstration Project for Yunnan’s Soil Remediation ( 2018BC004-2 ), the Science and Technology Major Project of Guangxi Province, China ( AA17204078 ) and Chinese Academy of Sciences .
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/1/5
Y1 - 2021/1/5
N2 - Silver nanoparticles (AgNPs) including a mix of intact nanoparticle-Ag and ‘free’ Ag+ pose high risks to benthic photoautotrophs, but the photosynthetic responses of benthic microbial aggregates to AgNPs still remain largely unknown. Here, periphyton and Nostoc were used to elucidate the photosynthetic responses of benthic algae community to intact nanoparticle-Ag and Ag+. During exposure, both intact nanoparticle-Ag and Ag+ imposed negative effects on photosynthesis of benthic algae, but via different pathways. Specifically, Ag+ had stronger effects on damaging the oxygen-evolving complex (OEC) and thylakoid membrane than intact nanoparticle-Ag. Ag+ also suppressed electron transfer from QA to QB, and impaired phycobilisome. Intact nanoparticle-Ag inhibited the expression of PsbD and PsbL in PSII, but prompted the ROS scavenging capacity. In response to the stress of AgNPs, the benthic algae increased light energy absorption to maintain the electron transport efficiency, and up-regulated PSI reaction center protein (PsaA) to compensate the degraded PSII. These results reveal how intact nanoparticle-Ag and Ag+ influence electron transport, energy conversion and protein expression in the photosynthesis of periphyton, and provide deep insights into the responses of benthic photoautotrophs to different components of AgNPs.
AB - Silver nanoparticles (AgNPs) including a mix of intact nanoparticle-Ag and ‘free’ Ag+ pose high risks to benthic photoautotrophs, but the photosynthetic responses of benthic microbial aggregates to AgNPs still remain largely unknown. Here, periphyton and Nostoc were used to elucidate the photosynthetic responses of benthic algae community to intact nanoparticle-Ag and Ag+. During exposure, both intact nanoparticle-Ag and Ag+ imposed negative effects on photosynthesis of benthic algae, but via different pathways. Specifically, Ag+ had stronger effects on damaging the oxygen-evolving complex (OEC) and thylakoid membrane than intact nanoparticle-Ag. Ag+ also suppressed electron transfer from QA to QB, and impaired phycobilisome. Intact nanoparticle-Ag inhibited the expression of PsbD and PsbL in PSII, but prompted the ROS scavenging capacity. In response to the stress of AgNPs, the benthic algae increased light energy absorption to maintain the electron transport efficiency, and up-regulated PSI reaction center protein (PsaA) to compensate the degraded PSII. These results reveal how intact nanoparticle-Ag and Ag+ influence electron transport, energy conversion and protein expression in the photosynthesis of periphyton, and provide deep insights into the responses of benthic photoautotrophs to different components of AgNPs.
KW - Ag
KW - AgNPs
KW - Algae
KW - iTRAQ quantitative proteomic analysis
KW - OJIP
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U2 - 10.1016/j.jhazmat.2020.123809
DO - 10.1016/j.jhazmat.2020.123809
M3 - Article
C2 - 33113741
AN - SCOPUS:85090940162
VL - 401
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
M1 - 123809
ER -