eMethylsorb: Electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

Abu Ali Ibn Sina, Sidney Howell, Laura G. Carrascosa, Sakandar Rauf, Muhammad J.A. Shiddiky, Matt Trau

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)

Abstract

We report a simple electrochemical method referred to as “eMethylsorb” for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.

Original languageEnglish
Pages (from-to)13153-13156
Number of pages4
JournalChemical Communications
Volume50
Issue number86
DOIs
Publication statusPublished - 02 Oct 2014

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