TY - GEN
T1 - Enabling rapid and specific SERS immunoassay using nano-scaled surface shear forces
AU - Vaidyanathan, Ramanathan
AU - Wang, Yuling
AU - Shiddiky, Muhammad J.A.
AU - Trau, Matt
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.
AB - A rapid and simple approach is presented to address the most critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD) - induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay based devices. These nanoscaled physical forces acting within nanometer distances from the surface enabled rapid (40 min), sensitive (10 fg/mL) and highly specific detection of human epidermal growth factor receptor 2 (HER2) in breast cancer patient samples.
KW - Immunoassay
KW - Nonspecific adsorption
KW - SERS
KW - Shear force
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M3 - Conference paper
AN - SCOPUS:84983316654
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 257
EP - 259
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -