TY - JOUR
T1 - Enhanced peroxidase mimetic activity of porous iron oxide nanoflakes
AU - Tanaka, Shunsuke
AU - Masud, Mostafa Kamal
AU - Kaneti, Yusuf Valentino
AU - Shiddiky, Muhammad J.A.
AU - Fatehmulla, Amanullah
AU - Aldhafiri, Abdullah M.
AU - Farooq, W. Aslam
AU - Bando, Yoshio
AU - Hossain, Md Shahriar A.
AU - Yamauchi, Yusuke
N1 - Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/4
Y1 - 2019/4
N2 - Porous nanomaterials with superior peroxidase mimetic activity (nanozyme) at room temperature have gained increasing attention as potential alternatives to natural peroxidase enzymes. Herein, we report the application of porous iron oxide nanoflakes (IONFs), synthesized using the combination of solvothermal method and high-temperature calcination as peroxidase nanozyme for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . The four IONF catalysts possess porous structures with a wide pore size distribution between 2–30 nm and high specific surface areas around to 200 m 2 g −1 . The increase of calcination temperature of the IONFs from 250 °C to 400 °C resulted in a gradual decrease in their specific surface area and Michaelis-Menten constant (K m ) for TMB oxidation. The optimum IONF sample showed a much lower K m at 0.24 mM (towards TMB) compared to natural enzyme horseradish peroxidase (HRP) at 0.434 mM, revealing the promising potential of the as-prepared IONFs as alternatives to HRP for biosensing applications.
AB - Porous nanomaterials with superior peroxidase mimetic activity (nanozyme) at room temperature have gained increasing attention as potential alternatives to natural peroxidase enzymes. Herein, we report the application of porous iron oxide nanoflakes (IONFs), synthesized using the combination of solvothermal method and high-temperature calcination as peroxidase nanozyme for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . The four IONF catalysts possess porous structures with a wide pore size distribution between 2–30 nm and high specific surface areas around to 200 m 2 g −1 . The increase of calcination temperature of the IONFs from 250 °C to 400 °C resulted in a gradual decrease in their specific surface area and Michaelis-Menten constant (K m ) for TMB oxidation. The optimum IONF sample showed a much lower K m at 0.24 mM (towards TMB) compared to natural enzyme horseradish peroxidase (HRP) at 0.434 mM, revealing the promising potential of the as-prepared IONFs as alternatives to HRP for biosensing applications.
KW - biosensors
KW - nanozymes
KW - peroxidase mimetic
KW - porous metal oxide
KW - two-dimensional nanostructures
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U2 - 10.1002/cnma.201800487
DO - 10.1002/cnma.201800487
M3 - Article
AN - SCOPUS:85061101900
SN - 2199-692X
VL - 5
SP - 506
EP - 513
JO - ChemNanoMat
JF - ChemNanoMat
IS - 4
ER -