The efficacy of the three fungi was tested in the laboratory on late instar nymphs of Z. butawengi. Spores of M. anisopliae and of P. lilacinum, and ground of H. citriformis were used as inocula and each fungus was applied at three concentrations of 1 x 104 , 1 x 105 and 1 x 106 spores per ml. Water and nil treatment were applied as controls. At the end of the experiment, the cadavers collected from the bioassay were isolated and identified to confirm Koch's postulates. The three fungi were successfully isolated from the test insects, however, the majority of the isolates were saprophytic fungi. The mortality data from the bioassay were analysed using binomial generalised linear mixed models. To get a general indication of how the fungal treatments performed, the effect of all fungal treatments were pooled and compared against the controls. Insect deaths by the pooled fungal treatment was not statistically different from the control treatments, however, the pooled fungal treatment killed test insects quicker than the controls. When the effects of individual concentrations were tested, there was statistical significant difference between their cumulative mortality indicating the different concentrations killed differently. The rate at which individual concentrations killed test insects also varied.
|Qualification||Master of Philosophy|
|Award date||31 Jan 2013|
|Place of Publication||Australia|
|Publication status||Published - 2013|