Environmental impact on biocontrol agents and secondary chemistry of Paterson's curse (Echium plantagineum)

Paul Weston, Leslie Weston, Shane Hildebrand

Research output: Book chapter/Published conference paperConference paper

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Abstract

Summary Geographically distinct populations ofPaterson's curse (Echium plantagineum L., Boragineacea),found near roadsides across NSW and VICwere surveyed along 3 distinct longitudinal transects inJuly/August and November/December 2011 for presenceof introduced biocontrol agents in shoots, rootsor flowers collected from sampled plants. Samplingwas conducted twice across 49 geographically distinctsites and associated biocontrol agents were identifiedand preserved. Survey results showed three of six possiblebiocontrol agents identified from collected plantspecimens, including a crown weevil, a root weeviland a flea beetle. Individual infestation varied acrosslocation and clustered distributions of each specieswere noted across NSW and VIC, possibly due toclimatic variation or proximity to former release sites.From each location, a composite sample of shootsand roots was collected. Root periderm extracts wereanalysed for naphthoquinone content spectrophotometricallyand by LC-ESI/MS. Shoot extracts weresubjected to solid phase extraction and LC-ESI/MSfor determination of pyrrolizidine alkaloids (PAs)and related N-oxides (PANOs). Metabolic profilingof 14 possible PAs and PANOs showed their consistentappearance in all shoot extracts with lepthamineN-oxide, echimidine-N oxide and echumine N-oxidepredominant. Root extracts contained shikonin, severalrelated naphthoquinones and two PANOs; contentvaried qualitatively and quantitatively with locationand time of sampling.Keywords Biocontrol agents, crown weevil,root weevil, flea beetle, roots, shoots, naphthoquinones,pyrrolizidine alkaloids, plant defence.
Original languageEnglish
Title of host publicationProceedings of the 18th Australasian Weeds Conference (2012)
Subtitle of host publicationDeveloping Solutions to Evolving Weed Problems
EditorsValerie Eldershaw
Place of PublicationMelbourne
PublisherWeed Society of Victoria
Pages203-207
Number of pages5
ISBN (Electronic)9780646586700
Publication statusPublished - 2012
Event18th Australasian Weeds Conference (2012) - The Sebel and Citigate Albert Park, Melbourne, Australia
Duration: 08 Oct 201211 Oct 2012
http://caws.org.au/awc_contents.php?yr=2012 (Conference proceedings)

Conference

Conference18th Australasian Weeds Conference (2012)
Abbreviated titleDeveloping Solutions to Evolving Weed Problems
CountryAustralia
CityMelbourne
Period08/10/1211/10/12
Internet address

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  • Cite this

    Weston, P., Weston, L., & Hildebrand, S. (2012). Environmental impact on biocontrol agents and secondary chemistry of Paterson's curse (Echium plantagineum). In V. Eldershaw (Ed.), Proceedings of the 18th Australasian Weeds Conference (2012): Developing Solutions to Evolving Weed Problems (pp. 203-207). Weed Society of Victoria.