Epithelial p53 gene expression and mutational analysis, combined with growth fraction assessment, in oral lichen planus

Mark Schifter, Alexandra M. Jones, D. Murray Walker

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The immunohistochemical detection of epithelial p53 protein expression in oral lichen planus (OLP) biopsies was supplemented with molecular analysis for mutations of the p53 gene using the polymerase chain reaction - single stranded conformational polymorphism (PCR-SSCP) technique. p53 protein expression, in the basal epithelial cell layer, as detected by the DO7 and 1801 antibodies, was significantly more frequent in OLP compared with other oral keratoses and normal mucosa, as was the growth fraction. The 10 OLP biopsies that had the most frequent p53 staining (plus a case of OLP found in continuity with a SCC) were screened by the PCR-SSCP technique, but no mutations were detected in the p53 gene (exons 5-9). The p53 overexpression in the OLP samples may be a physiological response to the hyper-proliferative state, as revealed by the growth fraction determination. This may usefully serve to protect against mutagenesis, and so be a factor in the low incidence of carcinoma associated with OLP.

Original languageEnglish
Pages (from-to)318-324
Number of pages7
JournalJournal Of Oral Pathology and Medicine
Volume27
Issue number7
Publication statusPublished - 01 Aug 1998
Externally publishedYes

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