Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation

H Zola; A Potter; S H Neoh; C A Juttner; D N Haylock; A M Rice; E J Favaloro; A Kabral; K F Bradstock

Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation

H Zola, A Potter, S H Neoh, C A Juttner, D N Haylock, A M Rice, E J Favaloro, A Kabral, K F Bradstock

Dentistry and Medical Sciences

Department of Clinical Immunology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A monoclonal antibody of the IgM class, reacting with the CD9 (p24) antigen is described. The antibody (FMC27) is cytotoxic against cells of the common type of acute lymphoblastic leukaemia (c-ALL), giving killing at higher dilutions than an IgG antibody (FMC8) against the same antigen. FMC27 and FMC8 recognise different epitopes, and FMC27 may thus be used in a cocktail together with FMC8 and an antibody against the c-ALL antigen, WM21. Furthermore, the IgM antibody can be coated directly onto magnetic microparticles for magnetic purging, unlike the IgG antibody which must be used in a two-layer procedure.

Original language	English
Pages (from-to)	297-301
Number of pages	5
Journal	Bone Marrow Transplantation
Volume	1
Issue number	3
Publication status	Published - Feb 1987

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{2db1348f8a4f427badb6eee5ec4ec748,

title = "Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation",

abstract = "A monoclonal antibody of the IgM class, reacting with the CD9 (p24) antigen is described. The antibody (FMC27) is cytotoxic against cells of the common type of acute lymphoblastic leukaemia (c-ALL), giving killing at higher dilutions than an IgG antibody (FMC8) against the same antigen. FMC27 and FMC8 recognise different epitopes, and FMC27 may thus be used in a cocktail together with FMC8 and an antibody against the c-ALL antigen, WM21. Furthermore, the IgM antibody can be coated directly onto magnetic microparticles for magnetic purging, unlike the IgG antibody which must be used in a two-layer procedure.",

keywords = "Antibodies, Monoclonal/therapeutic use, Antigen-Antibody Reactions, Antigens, CD, Antigens, Differentiation/immunology, Antilymphocyte Serum/toxicity, Bone Marrow/immunology, Bone Marrow Transplantation, Cell Line, Hematopoietic Stem Cells/immunology, Humans, Immunoglobulin M/therapeutic use, Leukemia, Myeloid, Acute/immunology, Lymphocyte Depletion, Membrane Glycoproteins, Precursor Cell Lymphoblastic Leukemia-Lymphoma/immunology, Tetraspanin 29, Transplantation, Autologous/methods",

author = "H Zola and A Potter and Neoh, {S H} and Juttner, {C A} and Haylock, {D N} and Rice, {A M} and Favaloro, {E J} and A Kabral and Bradstock, {K F}",

year = "1987",

month = feb,

language = "English",

volume = "1",

pages = "297--301",

journal = "Bone Marrow Transplantation",

issn = "0268-3369",

publisher = "Nature Publishing Group",

number = "3",

}

TY - JOUR

T1 - Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation

AU - Zola, H

AU - Potter, A

AU - Neoh, S H

AU - Juttner, C A

AU - Haylock, D N

AU - Rice, A M

AU - Favaloro, E J

AU - Kabral, A

AU - Bradstock, K F

PY - 1987/2

Y1 - 1987/2

N2 - A monoclonal antibody of the IgM class, reacting with the CD9 (p24) antigen is described. The antibody (FMC27) is cytotoxic against cells of the common type of acute lymphoblastic leukaemia (c-ALL), giving killing at higher dilutions than an IgG antibody (FMC8) against the same antigen. FMC27 and FMC8 recognise different epitopes, and FMC27 may thus be used in a cocktail together with FMC8 and an antibody against the c-ALL antigen, WM21. Furthermore, the IgM antibody can be coated directly onto magnetic microparticles for magnetic purging, unlike the IgG antibody which must be used in a two-layer procedure.

AB - A monoclonal antibody of the IgM class, reacting with the CD9 (p24) antigen is described. The antibody (FMC27) is cytotoxic against cells of the common type of acute lymphoblastic leukaemia (c-ALL), giving killing at higher dilutions than an IgG antibody (FMC8) against the same antigen. FMC27 and FMC8 recognise different epitopes, and FMC27 may thus be used in a cocktail together with FMC8 and an antibody against the c-ALL antigen, WM21. Furthermore, the IgM antibody can be coated directly onto magnetic microparticles for magnetic purging, unlike the IgG antibody which must be used in a two-layer procedure.

KW - Antibodies, Monoclonal/therapeutic use

KW - Antigen-Antibody Reactions

KW - Antigens, CD

KW - Antigens, Differentiation/immunology

KW - Antilymphocyte Serum/toxicity

KW - Bone Marrow/immunology

KW - Bone Marrow Transplantation

KW - Cell Line

KW - Hematopoietic Stem Cells/immunology

KW - Humans

KW - Immunoglobulin M/therapeutic use

KW - Leukemia, Myeloid, Acute/immunology

KW - Lymphocyte Depletion

KW - Membrane Glycoproteins

KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma/immunology

KW - Tetraspanin 29

KW - Transplantation, Autologous/methods

M3 - Article

C2 - 3332140

SN - 0268-3369

VL - 1

SP - 297

EP - 301

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

IS - 3

ER -

Evaluation of a monoclonal IgM antibody for purging of bone marrow for autologous transplantation

Abstract

UN SDGs

Fingerprint

Cite this