Seven sorghum accessions were evaluated quantitatively and qualitatively for the composition of their root exudates. Utilizing a unique capillary mat growing system, root exudates were collected from all sorghum accessions. Exudates were subjected to TLC and HPLC analysis to evaluate their chemical composition. Within each sorghum accession, variation existed in the amount of exudate produced and the chemical constituents of each exudate. Sorgoleone was the predominant constituent identified in each accession's exudate. Other closely related compounds, including 5-ethoxysorgoleone, 2,5-dimethoxysorgoleone, three other minor components (MW = 364, 388, and 402), and one unidentified component comprised the minor constituents of the root exudate. Our past work has shown that several of these compounds have potent phytotoxic activity as photosystem II inhibitors, thereby lending further support to the concept that Sorghum spp. are allelopathic and weed suppressive.