Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently not available. A total of 35 simple sequence repeat (SSR) primer pairs from potato (Solanum tuberosum), tomato (S. lycopersicum) and eggplant (S. melongena) were tested for cross-species transferability in S. elaeagnifolium. Among them, 13 primer pairs successfully produced alleles (bands). The polymorphism information content ranged from 0 to 0.84. The transferable rate of SSR from potato, tomato and eggplant to S. elaeagnifolium was 20%, 40% and 46% respectively. SSR analysis revealed high level of genetic diversity among 40 individuals collected within a paddock. Highly polymorphic and transferable cross-species SSR markers would be useful for determining the extent of genetic diversity in S. elaeagnifolium populations.