Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for silverleaf nightshade (Solanum elaeagnifolium Cav.)

Xiaocheng Zhu; Hanwen Wu; Harsh Raman; Deirdre Lemerle; Rex Stanton; Geoffrey Burrows

doi:10.1111/j.1365-3180.2012.00908.x

Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for silverleaf nightshade (Solanum elaeagnifolium Cav.)

Xiaocheng Zhu, Hanwen Wu, Harsh Raman, Deirdre Lemerle, Rex Stanton, Geoffrey Burrows

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently not available. A total of 35 simple sequence repeat (SSR) primer pairs from potato (Solanum tuberosum), tomato (S. lycopersicum) and eggplant (S. melongena) were tested for cross-species transferability in S. elaeagnifolium. Among them, 13 primer pairs successfully produced alleles (bands). The polymorphism information content ranged from 0 to 0.84. The transferable rate of SSR from potato, tomato and eggplant to S. elaeagnifolium was 20%, 40% and 46% respectively. SSR analysis revealed high level of genetic diversity among 40 individuals collected within a paddock. Highly polymorphic and transferable cross-species SSR markers would be useful for determining the extent of genetic diversity in S. elaeagnifolium populations.

Original language	English
Pages (from-to)	21-223
Number of pages	203
Journal	Weed Research
Volume	52
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-3180.2012.00908.x
Publication status	Published - Jun 2012

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10.1111/j.1365-3180.2012.00908.x

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title = "Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for silverleaf nightshade (Solanum elaeagnifolium Cav.)",

abstract = "Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently not available. A total of 35 simple sequence repeat (SSR) primer pairs from potato (Solanum tuberosum), tomato (S. lycopersicum) and eggplant (S. melongena) were tested for cross-species transferability in S. elaeagnifolium. Among them, 13 primer pairs successfully produced alleles (bands). The polymorphism information content ranged from 0 to 0.84. The transferable rate of SSR from potato, tomato and eggplant to S. elaeagnifolium was 20%, 40% and 46% respectively. SSR analysis revealed high level of genetic diversity among 40 individuals collected within a paddock. Highly polymorphic and transferable cross-species SSR markers would be useful for determining the extent of genetic diversity in S. elaeagnifolium populations.",

keywords = "Cross-species SSR, Genetic diversity, Invasive weed, Microsatellite, Silverleaf nightshade",

author = "Xiaocheng Zhu and Hanwen Wu and Harsh Raman and Deirdre Lemerle and Rex Stanton and Geoffrey Burrows",

note = "Imported on 12 Apr 2017 - DigiTool details were: month (773h) = June, 2012; Journal title (773t) = Weed Research. ISSNs: 0043-1737; ",

year = "2012",

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doi = "10.1111/j.1365-3180.2012.00908.x",

language = "English",

volume = "52",

pages = "21--223",

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T1 - Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for silverleaf nightshade (Solanum elaeagnifolium Cav.)

AU - Zhu, Xiaocheng

AU - Wu, Hanwen

AU - Raman, Harsh

AU - Lemerle, Deirdre

AU - Stanton, Rex

AU - Burrows, Geoffrey

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = June, 2012; Journal title (773t) = Weed Research. ISSNs: 0043-1737;

PY - 2012/6

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N2 - Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently not available. A total of 35 simple sequence repeat (SSR) primer pairs from potato (Solanum tuberosum), tomato (S. lycopersicum) and eggplant (S. melongena) were tested for cross-species transferability in S. elaeagnifolium. Among them, 13 primer pairs successfully produced alleles (bands). The polymorphism information content ranged from 0 to 0.84. The transferable rate of SSR from potato, tomato and eggplant to S. elaeagnifolium was 20%, 40% and 46% respectively. SSR analysis revealed high level of genetic diversity among 40 individuals collected within a paddock. Highly polymorphic and transferable cross-species SSR markers would be useful for determining the extent of genetic diversity in S. elaeagnifolium populations.

AB - Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently not available. A total of 35 simple sequence repeat (SSR) primer pairs from potato (Solanum tuberosum), tomato (S. lycopersicum) and eggplant (S. melongena) were tested for cross-species transferability in S. elaeagnifolium. Among them, 13 primer pairs successfully produced alleles (bands). The polymorphism information content ranged from 0 to 0.84. The transferable rate of SSR from potato, tomato and eggplant to S. elaeagnifolium was 20%, 40% and 46% respectively. SSR analysis revealed high level of genetic diversity among 40 individuals collected within a paddock. Highly polymorphic and transferable cross-species SSR markers would be useful for determining the extent of genetic diversity in S. elaeagnifolium populations.

KW - Cross-species SSR

KW - Genetic diversity

KW - Invasive weed

KW - Microsatellite

KW - Silverleaf nightshade

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DO - 10.1111/j.1365-3180.2012.00908.x

M3 - Article

SN - 0043-1737

VL - 52

SP - 21

EP - 223

JO - Weed Research

JF - Weed Research

IS - 3

ER -

Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for silverleaf nightshade (Solanum elaeagnifolium Cav.)

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