Galectin-11 and galectin-14 are ruminant galectins involved in parasiticinfections. Although their roles in parasite immunity are still beingelucidated, it appears that their functions are parasite specific. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. This chapter discusses the methods designed to study parasitic infections in sheep, which have provided us with insight into the functions of galectin-11 and galectin-14 during host' parasite interactions. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins and in vitro assays to monitor the effect of galectins on larval development.
|Title of host publication||Galectins|
|Subtitle of host publication||Methods and protocols|
|Editors||S. Stowell, R. Cummings|
|Place of Publication||New York|
|Number of pages||25|
|Publication status||Published - 2015|
Preston, S., Dunphy, J., Beddoe, T., Meeusen, E., & Young, A. (2015). Evaluation of the Role of Galectins in Parasite Immunity. In S. Stowell, & R. Cummings (Eds.), Galectins: Methods and protocols (25 ed., pp. 371-395). Humana Press.