Exploring the Fasciola hepatica tegument proteome

R. Alan Wilson, Janelle Wright, William de Castro-Borges, Sophie J. Parker-manuel, Adam A. Dowle, Peter D. Ashton, Neil D. Young, B. Gasser Robin, Terry Spithill

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The surface tegument of the liver fluke Fasciola hepatica is a syncytial cytoplasmic layer bounded externally by a plasma membrane and covered by a glycocalyx, which constitutes the interface between the parasite and its ruminant host. The tegument's interaction with the immune system during the fluke's protracted migration from the gut lumen through the peritoneal cavity and liver parenchyma to the lumen of the bile duct, plays a key role in the fluke's establishment or elimination. However, little is known about proteins of the tegument surface or its secretions. We applied techniques developed for the blood fluke, Schistosoma mansoni, to enrich a tegument surface membrane preparation and analyse its composition by tandem mass spectrometry using new transcript databases for F. hepatica. We increased the membrane and secretory pathway components of the final preparation to ~30%, whilst eliminating contaminating proteases. We identified a series of proteins or transcripts shared with the schistosome tegument including annexins, a tetraspanin, carbonic anhydrase and an orthologue of a host protein (CD59) that inhibits complement fixation. Unique to F. hepatica, we also found proteins with lectin, cubulin and von Willebrand factor domains plus 10 proteins with leader sequences or transmembrane helices. Many of these surface proteins are potential vaccine candidates. We were hampered in collecting tegument secretions by the propensity of liver flukes, unlike blood flukes, to vomit their gut contents. We analysed both the 'vomitus' and a second supernatant released from haematin-depleted flukes. We identified many proteases, some novel, as well as a second protein with a von Willebrand factor domain. This study demonstrates that components of the tegumental surface of F. hepatica can be defined using proteomic approaches, but also indicates the need to prevent vomiting if tegument secretions are to be characterised.
Original languageEnglish
Pages (from-to)1347-1359
Number of pages13
JournalInternational Journal for Parasitology
Volume41
Issue number13-14
DOIs
Publication statusPublished - May 2011

Fingerprint

Fasciola hepatica
Proteome
Trematoda
von Willebrand Factor
Proteins
Membrane Proteins
Peptide Hydrolases
Secretory Component
Annexins
Glycocalyx
Hemin
Carbonic Anhydrases
Membranes
Schistosoma mansoni
Secretory Pathway
Peritoneal Cavity
Ruminants
Tandem Mass Spectrometry
Bile Ducts
Lectins

Cite this

Alan Wilson, R., Wright, J., de Castro-Borges, W., Parker-manuel, S. J., Dowle, A. A., Ashton, P. D., ... Spithill, T. (2011). Exploring the Fasciola hepatica tegument proteome. International Journal for Parasitology, 41(13-14), 1347-1359. https://doi.org/10.1016/j.ijpara.2011.08.003
Alan Wilson, R. ; Wright, Janelle ; de Castro-Borges, William ; Parker-manuel, Sophie J. ; Dowle, Adam A. ; Ashton, Peter D. ; Young, Neil D. ; Robin, B. Gasser ; Spithill, Terry. / Exploring the Fasciola hepatica tegument proteome. In: International Journal for Parasitology. 2011 ; Vol. 41, No. 13-14. pp. 1347-1359.
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Alan Wilson, R, Wright, J, de Castro-Borges, W, Parker-manuel, SJ, Dowle, AA, Ashton, PD, Young, ND, Robin, BG & Spithill, T 2011, 'Exploring the Fasciola hepatica tegument proteome', International Journal for Parasitology, vol. 41, no. 13-14, pp. 1347-1359. https://doi.org/10.1016/j.ijpara.2011.08.003

Exploring the Fasciola hepatica tegument proteome. / Alan Wilson, R.; Wright, Janelle; de Castro-Borges, William; Parker-manuel, Sophie J.; Dowle, Adam A.; Ashton, Peter D.; Young, Neil D.; Robin, B. Gasser; Spithill, Terry.

In: International Journal for Parasitology, Vol. 41, No. 13-14, 05.2011, p. 1347-1359.

Research output: Contribution to journalArticle

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KW - Vomitus

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Alan Wilson R, Wright J, de Castro-Borges W, Parker-manuel SJ, Dowle AA, Ashton PD et al. Exploring the Fasciola hepatica tegument proteome. International Journal for Parasitology. 2011 May;41(13-14):1347-1359. https://doi.org/10.1016/j.ijpara.2011.08.003