Expression patterns of genes encoding sugar and potassium transport proteins are simultaneously upregulated or downregulated when carbon and potassium availability is modified in shiraz (Vitis vinifera L.) berries

Zelmari A. Coetzee, Rob R. Walker, Siyang Liao, Celia Barril, Alain J. Deloire, Simon J. Clarke, Stephen D. Tyerman, Suzy Y. Rogiers

Research output: Contribution to journalArticle

Abstract

A link between the accumulation of sugar and potassium has previously been described for ripening grape berries. The functional basis of this link has, as of yet, not been elucidated but could potentially be associated with the integral role that potassium has in phloem transport. An experiment was conducted on Shiraz grapevines in a controlled environment. The accumulation of berry sugar was curtailed by reducing the leaf photoassimilation rate, and the availability of potassium was increased through soil fertilization. The study characterizes the relationship between the accumulation of sugar and potassium into the grape berry and describes how their accumulation patterns are related to the expression patterns of their transporter proteins. A strong connection was observed between the accumulation of sugar and potassium in the grape berry pericarp, irrespective of the treatment. The relative expression of proteins associated with sugar and potassium transport across the tonoplast and plasma membrane was closely correlated, suggesting transcriptional coregulation leading to the simultaneous translocation and storage of potassium and sugar in the grape berry cell.

Original languageEnglish
Pages (from-to)2331-2342
Number of pages12
JournalPlant & cell physiology
Volume60
Issue number10
Early online date10 Jul 2019
DOIs
Publication statusPublished - 01 Oct 2019

Fingerprint

Vitis
transport proteins
Vitis vinifera
small fruits
Fruit
Potassium
Carrier Proteins
Carbon
Down-Regulation
potassium
sugars
Gene Expression
carbon
grapes
genes
Phloem
Controlled Environment
tonoplast
pericarp
Fertilization

Cite this

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title = "Expression patterns of genes encoding sugar and potassium transport proteins are simultaneously upregulated or downregulated when carbon and potassium availability is modified in shiraz (Vitis vinifera L.) berries",
abstract = "A link between the accumulation of sugar and potassium has previously been described for ripening grape berries. The functional basis of this link has, as of yet, not been elucidated but could potentially be associated with the integral role that potassium has in phloem transport. An experiment was conducted on Shiraz grapevines in a controlled environment. The accumulation of berry sugar was curtailed by reducing the leaf photoassimilation rate, and the availability of potassium was increased through soil fertilization. The study characterizes the relationship between the accumulation of sugar and potassium into the grape berry and describes how their accumulation patterns are related to the expression patterns of their transporter proteins. A strong connection was observed between the accumulation of sugar and potassium in the grape berry pericarp, irrespective of the treatment. The relative expression of proteins associated with sugar and potassium transport across the tonoplast and plasma membrane was closely correlated, suggesting transcriptional coregulation leading to the simultaneous translocation and storage of potassium and sugar in the grape berry cell.",
keywords = "Vitis vinifera, Gene expression, Grape berry pericarp, Potassium, Sugar, Transport proteins",
author = "Coetzee, {Zelmari A.} and Walker, {Rob R.} and Siyang Liao and Celia Barril and Deloire, {Alain J.} and Clarke, {Simon J.} and Tyerman, {Stephen D.} and Rogiers, {Suzy Y.}",
year = "2019",
month = "10",
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doi = "10.1093/pcp/pcz130",
language = "English",
volume = "60",
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journal = "Plant and Cell Physiology",
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TY - JOUR

T1 - Expression patterns of genes encoding sugar and potassium transport proteins are simultaneously upregulated or downregulated when carbon and potassium availability is modified in shiraz (Vitis vinifera L.) berries

AU - Coetzee, Zelmari A.

AU - Walker, Rob R.

AU - Liao, Siyang

AU - Barril, Celia

AU - Deloire, Alain J.

AU - Clarke, Simon J.

AU - Tyerman, Stephen D.

AU - Rogiers, Suzy Y.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - A link between the accumulation of sugar and potassium has previously been described for ripening grape berries. The functional basis of this link has, as of yet, not been elucidated but could potentially be associated with the integral role that potassium has in phloem transport. An experiment was conducted on Shiraz grapevines in a controlled environment. The accumulation of berry sugar was curtailed by reducing the leaf photoassimilation rate, and the availability of potassium was increased through soil fertilization. The study characterizes the relationship between the accumulation of sugar and potassium into the grape berry and describes how their accumulation patterns are related to the expression patterns of their transporter proteins. A strong connection was observed between the accumulation of sugar and potassium in the grape berry pericarp, irrespective of the treatment. The relative expression of proteins associated with sugar and potassium transport across the tonoplast and plasma membrane was closely correlated, suggesting transcriptional coregulation leading to the simultaneous translocation and storage of potassium and sugar in the grape berry cell.

AB - A link between the accumulation of sugar and potassium has previously been described for ripening grape berries. The functional basis of this link has, as of yet, not been elucidated but could potentially be associated with the integral role that potassium has in phloem transport. An experiment was conducted on Shiraz grapevines in a controlled environment. The accumulation of berry sugar was curtailed by reducing the leaf photoassimilation rate, and the availability of potassium was increased through soil fertilization. The study characterizes the relationship between the accumulation of sugar and potassium into the grape berry and describes how their accumulation patterns are related to the expression patterns of their transporter proteins. A strong connection was observed between the accumulation of sugar and potassium in the grape berry pericarp, irrespective of the treatment. The relative expression of proteins associated with sugar and potassium transport across the tonoplast and plasma membrane was closely correlated, suggesting transcriptional coregulation leading to the simultaneous translocation and storage of potassium and sugar in the grape berry cell.

KW - Vitis vinifera

KW - Gene expression

KW - Grape berry pericarp

KW - Potassium

KW - Sugar

KW - Transport proteins

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