Fabrication of ultra-thin polypyrrole-glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose

Samuel B. Adeloju, Alexander N. Moline

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

A simple electropolymerisation process is described for the fabrication of an ultra-thin (∼55 nm) polypyrrole (PPy)-glucose oxidase (GOD) film in a supporting electrolyte-free monomer solution for potentiometric biosensing of glucose. The optimum conditions for growing the ultra-thin film include 0.1 M pyrrole, 55-110 U/ml GOD, an applied current density of 0.05 mA/cm2 and an electrical charge of 25 mC/cm2. Long-term storage of the biosensor in acetate buffer improved the sensitivity of the biosensor by a factor of approximately two. The biosensor can also be used repeatedly for over 2 months with little or no loss in sensitivity. The interference effect of ascorbic acid was successfully reduced by inclusion of an outer PPy-Cl layer.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalBiosensors and Bioelectronics
Volume16
Issue number3
DOIs
Publication statusPublished - 01 May 2001

Fingerprint Dive into the research topics of 'Fabrication of ultra-thin polypyrrole-glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose'. Together they form a unique fingerprint.

Cite this