Abstract
Background: The Emergence of plasmid-mediated colistin resistance in poultry origin Escherichia coli has become a major global concern. As colistin has also been being used in breeder chicken for several years, we conducted this study in 108 breeder farms of Bangladesh to unveil the present prevalence and molecular basis of colistin-resistance of E. coli (CREC) in breeder poultry.
Methods and materials: During a one year study period, following standard wet-lab procedure, we isolated 24 (26.1%; 95%CI: 18.2–35.9) CREC among which 62.5% (95%CI: 38.5–81.6) isolates were confirmed, by both simplex and multiplex polymerase chain reaction (PCR), carrying one or more mcr gene(s).
Results: In PCR, the mcr-1, mcr-2 and mcr-3 were detected in eight (50%; 95%CI: 28–72). one (6.25%; 95%CI: Conclusion: As per our knowledge, it is the first report on the detection and sequencing of mcr-1 gene of E. coli isolated from breeder chicken. The findings of this study clarify the emergence of CREC in breeder chicken; and recommend the reduction of colistin usage along with adoption of an on-time surveillance system in poultry farms.
Methods and materials: During a one year study period, following standard wet-lab procedure, we isolated 24 (26.1%; 95%CI: 18.2–35.9) CREC among which 62.5% (95%CI: 38.5–81.6) isolates were confirmed, by both simplex and multiplex polymerase chain reaction (PCR), carrying one or more mcr gene(s).
Results: In PCR, the mcr-1, mcr-2 and mcr-3 were detected in eight (50%; 95%CI: 28–72). one (6.25%; 95%CI: Conclusion: As per our knowledge, it is the first report on the detection and sequencing of mcr-1 gene of E. coli isolated from breeder chicken. The findings of this study clarify the emergence of CREC in breeder chicken; and recommend the reduction of colistin usage along with adoption of an on-time surveillance system in poultry farms.
| Original language | English |
|---|---|
| Pages | 17-17 |
| Number of pages | 1 |
| DOIs | |
| Publication status | Published - 16 Nov 2020 |
| Event | 19th International Congress on Infectious Diseases - Kuala Lumpur Convention Centre , Kuala Lumpur , Malaysia Duration: 10 Sept 2020 → 13 Sept 2020 https://www.goinginternational.eu/wp/de/19th-international-congress-on-infectious-diseases-19th-icid/ https://www.ijidonline.com/article/S1201-9712(20)32308-0/pdf |
Conference
| Conference | 19th International Congress on Infectious Diseases |
|---|---|
| Country/Territory | Malaysia |
| City | Kuala Lumpur |
| Period | 10/09/20 → 13/09/20 |
| Other | CANCELLED DUE TO COVID 19 The International Society for Infectious Diseases (ISID) is collaborating with the Malaysian Society of Infectious Diseases and Chemotherapy (MSIDC) for the 19th International Congress on Infectious Diseases (ICID).The 19th ICID features an expanded range of sessions by bringing the One Health aspect to the world of clinical infectious diseases and microbiology. |
| Internet address |