TY - JOUR
T1 - Focusing in on structural genomics
T2 - the University of Queensland structural biology pipeline
AU - Puri, M.
AU - Robin, G.
AU - Cowieson, N.
AU - Forwood, Jade
AU - Listwan, P.
AU - Hu, S. H.
AU - Guncar, G.
AU - Huber, T.
AU - Kellie, Stuart
AU - Hume, David A
AU - Kobe, Bostjan
AU - Martin, Jennifer, L.
N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = New Biotechnology. ISSNs: 1389-0344;
PY - 2006
Y1 - 2006
N2 - The flood of new genomic sequence information together with technological innovations in protein structure determination have led to worldwide structural genomics (SG) initiatives. The goals of SG initiatives are to accelerate the process of protein structure determination, to fill in protein fold space and to provide information about the function of uncharacterized proteins. In the long-term, these outcomes are likely to impact on medical biotechnology and drug discovery, leading to a better understanding of disease as well as the development of new therapeutics. Here we describe the high throughput pipeline established at the University of Queensland in Australia. In this focused pipeline, the targets for structure determination are proteins that are expressed in mouse macrophage cells and that are inferred to have a role in innate immunity. The aim is to characterize the molecular structure and the biochemical and cellular function of these targets by using a parallel processing pipeline. The pipeline is designed to work with tens to hundreds of target gene products and comprises target selection, cloning, expression, purification, crystallization and structure determination. The structures from this pipeline will provide insights into the function of previously uncharacterized macrophage proteins and could lead to the validation of new drug targets for chronic obstructive pulmonary disease and arthritis. (c) 2006 Elsevier B.V. All rights reserved.
AB - The flood of new genomic sequence information together with technological innovations in protein structure determination have led to worldwide structural genomics (SG) initiatives. The goals of SG initiatives are to accelerate the process of protein structure determination, to fill in protein fold space and to provide information about the function of uncharacterized proteins. In the long-term, these outcomes are likely to impact on medical biotechnology and drug discovery, leading to a better understanding of disease as well as the development of new therapeutics. Here we describe the high throughput pipeline established at the University of Queensland in Australia. In this focused pipeline, the targets for structure determination are proteins that are expressed in mouse macrophage cells and that are inferred to have a role in innate immunity. The aim is to characterize the molecular structure and the biochemical and cellular function of these targets by using a parallel processing pipeline. The pipeline is designed to work with tens to hundreds of target gene products and comprises target selection, cloning, expression, purification, crystallization and structure determination. The structures from this pipeline will provide insights into the function of previously uncharacterized macrophage proteins and could lead to the validation of new drug targets for chronic obstructive pulmonary disease and arthritis. (c) 2006 Elsevier B.V. All rights reserved.
KW - Applied microbiology, genetics
KW - Biochemical research methods, biotechnology
KW - Heredity, high throughput crystallography, protein expression, macrophage proteins, crystallization, structural genomics, thermotoga-maritima proteome, drug discovery, crystal-growth, functional genomics, membrane-proteins, escherichia-coli, crystallizati
U2 - 10.1016/j.bioeng.2006.09.002
DO - 10.1016/j.bioeng.2006.09.002
M3 - Article
C2 - 17097918
VL - 23
SP - 281
EP - 289
JO - Biomolecular Engineering
JF - Biomolecular Engineering
SN - 1389-0344
IS - 6
ER -