Formation of bitter peptides during ripening of ovine milk cheese made with different coagulants

Samson Agboola, ShaoJiang Chen, Jian Zhao

Research output: Contribution to journalArticle

18 Citations (Scopus)
1 Downloads (Pure)

Abstract

Hydrophilic and hydrophobic peptides in the water-soluble fraction of semi-hard, vacuum packed ovine cheeses made with calf, microbial and cardoon coagulants were monitored for 90 d by reversed-phase HPLC. Hydrophobic peptides of the cheeses were further analysed by size exclusion HPLC to determine their molecular sizes and the bitterness of the cheeses was evaluated by a trained sensory panel. Cheese made with cardoon coagulant had the highest levels of both hydrophilic and hydrophobic peptides. Cheeses made with calf and microbial coagulants had comparable levels of hydrophilic peptides, but the former had higher levels of hydrophobic peptides. The concentration of bitter peptides (those with a molecular size of 165-6500 g·mol-1) was highest in cheese made with microbial coagulant and lowest in cheese made with calf rennet. Cheese made with microbial coagulant was perceived to be the most bitter by the sensory panel, followed by calf and cardoon coagulant cheeses. Sensory bitterness score was significantly (P < 0.05) correlated with total bitter peptides and the ratio of bitter peptides to total peptides, but not with total hydrophobic peptides.
Original languageEnglish
Pages (from-to)567-578
Number of pages12
JournalDairy Science and Technology
Volume84
Issue number6
DOIs
Publication statusPublished - 2004

Fingerprint

Cheeses
Coagulants
coagulants
ewe milk
Cheese
cheeses
Sheep
Milk
ripening
peptides
Peptides
Cynara
calves
bitterness
High Pressure Liquid Chromatography
rennet
reversed-phase high performance liquid chromatography
Vacuum
high performance liquid chromatography
sheep

Cite this

Agboola, Samson ; Chen, ShaoJiang ; Zhao, Jian. / Formation of bitter peptides during ripening of ovine milk cheese made with different coagulants. In: Dairy Science and Technology. 2004 ; Vol. 84, No. 6. pp. 567-578.
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abstract = "Hydrophilic and hydrophobic peptides in the water-soluble fraction of semi-hard, vacuum packed ovine cheeses made with calf, microbial and cardoon coagulants were monitored for 90 d by reversed-phase HPLC. Hydrophobic peptides of the cheeses were further analysed by size exclusion HPLC to determine their molecular sizes and the bitterness of the cheeses was evaluated by a trained sensory panel. Cheese made with cardoon coagulant had the highest levels of both hydrophilic and hydrophobic peptides. Cheeses made with calf and microbial coagulants had comparable levels of hydrophilic peptides, but the former had higher levels of hydrophobic peptides. The concentration of bitter peptides (those with a molecular size of 165-6500 g{\^A}·mol-1) was highest in cheese made with microbial coagulant and lowest in cheese made with calf rennet. Cheese made with microbial coagulant was perceived to be the most bitter by the sensory panel, followed by calf and cardoon coagulant cheeses. Sensory bitterness score was significantly (P < 0.05) correlated with total bitter peptides and the ratio of bitter peptides to total peptides, but not with total hydrophobic peptides.",
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Formation of bitter peptides during ripening of ovine milk cheese made with different coagulants. / Agboola, Samson; Chen, ShaoJiang; Zhao, Jian.

In: Dairy Science and Technology, Vol. 84, No. 6, 2004, p. 567-578.

Research output: Contribution to journalArticle

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T1 - Formation of bitter peptides during ripening of ovine milk cheese made with different coagulants

AU - Agboola, Samson

AU - Chen, ShaoJiang

AU - Zhao, Jian

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