Functional analysis of rice genes determining fragrance

Robert J Henry; Louis MT Bradbury; Timothy L Fitzgerald; Susan A Gillies; Nigel Slater; Daniel LE Waters

Functional analysis of rice genes determining fragrance

Robert J Henry, Louis MT Bradbury, Timothy L Fitzgerald, Susan A Gillies, Nigel Slater, Daniel LE Waters

Research output: Other contribution to conference › Other › peer-review

Abstract

The fragrance of rice has been associated with a deletion resulting in the apparent loss of function of a gene annotated as a betaine aldehyde dehydrogenase (BAD2) on chromosome 8. We have now compared the levels of expression of BAD2 and BAD1 (chromosome 4) from fragrant and non fragrant genotypes. Expression of BAD2 predominates in non fragrant genotypes but is reduced in genotypes with the fragrance allele. Expression of BAD1 and BAD2 proteins in bacteria was used to test the specificity of the reactions catalysed by these enzymes. This analysis suggests that BAD2 encodes a 4-aminobutyraldehyde dehydrogenase. Analysis of transgenic rice plants over-expressing these genes is in progress.

Original language	Undefined/Unknown
Publication status	Published - 2007
Event	5th Rice Functional Genomics Symposium - Duration: 15 Oct 2007 → …

Conference

Conference	5th Rice Functional Genomics Symposium
Period	15/10/07 → …

Access to Document

http://epubs.scu.edu.au/cpcg_pubs/202/

Cite this

@conference{ef8addc202474b618d82a2f4d5ce3275,

title = "Functional analysis of rice genes determining fragrance",

abstract = "The fragrance of rice has been associated with a deletion resulting in the apparent loss of function of a gene annotated as a betaine aldehyde dehydrogenase (BAD2) on chromosome 8. We have now compared the levels of expression of BAD2 and BAD1 (chromosome 4) from fragrant and non fragrant genotypes. Expression of BAD2 predominates in non fragrant genotypes but is reduced in genotypes with the fragrance allele. Expression of BAD1 and BAD2 proteins in bacteria was used to test the specificity of the reactions catalysed by these enzymes. This analysis suggests that BAD2 encodes a 4-aminobutyraldehyde dehydrogenase. Analysis of transgenic rice plants over-expressing these genes is in progress.",

author = "Henry, {Robert J} and Bradbury, {Louis MT} and Fitzgerald, {Timothy L} and Gillies, {Susan A} and Nigel Slater and Waters, {Daniel LE}",

year = "2007",

language = "Undefined/Unknown",

note = "5th Rice Functional Genomics Symposium ; Conference date: 15-10-2007",

}

TY - CONF

T1 - Functional analysis of rice genes determining fragrance

AU - Henry, Robert J

AU - Bradbury, Louis MT

AU - Fitzgerald, Timothy L

AU - Gillies, Susan A

AU - Slater, Nigel

AU - Waters, Daniel LE

PY - 2007

Y1 - 2007

N2 - The fragrance of rice has been associated with a deletion resulting in the apparent loss of function of a gene annotated as a betaine aldehyde dehydrogenase (BAD2) on chromosome 8. We have now compared the levels of expression of BAD2 and BAD1 (chromosome 4) from fragrant and non fragrant genotypes. Expression of BAD2 predominates in non fragrant genotypes but is reduced in genotypes with the fragrance allele. Expression of BAD1 and BAD2 proteins in bacteria was used to test the specificity of the reactions catalysed by these enzymes. This analysis suggests that BAD2 encodes a 4-aminobutyraldehyde dehydrogenase. Analysis of transgenic rice plants over-expressing these genes is in progress.

AB - The fragrance of rice has been associated with a deletion resulting in the apparent loss of function of a gene annotated as a betaine aldehyde dehydrogenase (BAD2) on chromosome 8. We have now compared the levels of expression of BAD2 and BAD1 (chromosome 4) from fragrant and non fragrant genotypes. Expression of BAD2 predominates in non fragrant genotypes but is reduced in genotypes with the fragrance allele. Expression of BAD1 and BAD2 proteins in bacteria was used to test the specificity of the reactions catalysed by these enzymes. This analysis suggests that BAD2 encodes a 4-aminobutyraldehyde dehydrogenase. Analysis of transgenic rice plants over-expressing these genes is in progress.

M3 - Other

T2 - 5th Rice Functional Genomics Symposium

Y2 - 15 October 2007

ER -