Functional enrichment of mannanase-treated spent brewer yeast

Ruge Cao, Xingyue Yang, Wenting Shang, Zhongkai Zhou, Padraig Strappe, Chris Blanchard

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


In this study, the effect of Bacillus amyloliquefaciens-produced β-mannanase on the nutrient diffusion (release) and antioxidant activity of spent brewer yeast (SBY) was investigated. Three pretreatments were performed: (1) autolysis at 50°C for 24 h; (2) autolysis at 50°C for 24 h, with the addition of β-mannanase during the autolysis; (3) autolysis at 50°C for 24 h, and the β-mannanase was added for another 12 h treatment. The pretreatments with the addition of β-mannanase caused significant cell wall degradation, markedly increased the yield of SBY extracts. More importantly, this study found that polysaccharides were degraded to be oligosaccharides with a considerable reduction in molecular weights. Meanwhile, pretreatment with the enzyme also exhibited a higher antioxidant activity in SBY extract compared to autolysis itself. The current study indicated that pretreatment (3) had a better effect than pretreatment (2) in terms of improving in antioxidant activity in SBY extract. These improved characteristics of SBY extracts isolated through enzymatic treatment appear to show promising features for their prospective use as natural functional agents.
Original languageEnglish
Pages (from-to) 789-794
Number of pages6
JournalPreparative Biochemistry and Biotechnology
Issue number8
Early online dateJun 2017
Publication statusPublished - Jul 2017


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