Galvanostatic entrapment of penicillinase into polytyramine films and its utilization for the potentiometric determination of penicillin

Fatma Ismail, Samuel B. Adeloju

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)
5 Downloads (Pure)

Abstract

A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3, and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3-283 μM for penicillin and achieved a minimum detectable concentration of 0.3 μM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ± 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm.

Original languageEnglish
Pages (from-to)2851-2868
Number of pages18
JournalSensors
Volume10
Issue number4
DOIs
Publication statusPublished - 01 Apr 2010

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