Genetic differentiation, diversity, and drug susceptibility of Candida Krusei

Jie Gong, Meng Xiao, He Wang, Timothy Kudinha, Yu Wang, Fei Zhao, Weiwei Wu, Lihua He, Ying Chun Xu, Jianzhong Zhang

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Abstract

Candida krusei is a notable pathogenic fungus that causes invasive candidiasis, mainly due to its natural resistance to fluconazole. However, to date, there is limited research on the genetic population features of C. krusei. We developed a set of microsatellite markers for this organism, with a cumulative discriminatory power of 1,000. Using these microsatellite loci, 48 independent C. krusei strains of clearly known the sources, were analyzed. Furthermore, susceptibility to 9 antifungal agents was determined for each strain, by the Clinical and Laboratory Standards Institute broth microdilution method. Population structure analyses revealed that C. krusei could be separated into two clusters. The cluster with the higher genetic diversity had wider MIC ranges for six antifungal agents. Furthermore, the highest MIC values of the six antifungal agents belonged to the cluster with higher genetic diversity. The higher genetic diversity cluster might have a better adaptive capacity when C. krusei is under selection pressure from antifungal agents, and thus is more likely to develop drug resistance.

Original languageEnglish
Article number2717
Pages (from-to)1-10
Number of pages10
JournalFrontiers in Microbiology
Volume9
Issue numberNOV
DOIs
Publication statusPublished - 20 Nov 2018

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Antifungal Agents
Candida
Pharmaceutical Preparations
Microsatellite Repeats
Invasive Candidiasis
Genetic Research
Fluconazole
Population Genetics
Drug Resistance
Innate Immunity
Fungi
Pressure
Population

Cite this

Gong, Jie ; Xiao, Meng ; Wang, He ; Kudinha, Timothy ; Wang, Yu ; Zhao, Fei ; Wu, Weiwei ; He, Lihua ; Xu, Ying Chun ; Zhang, Jianzhong. / Genetic differentiation, diversity, and drug susceptibility of Candida Krusei. In: Frontiers in Microbiology. 2018 ; Vol. 9, No. NOV. pp. 1-10.
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Gong, J, Xiao, M, Wang, H, Kudinha, T, Wang, Y, Zhao, F, Wu, W, He, L, Xu, YC & Zhang, J 2018, 'Genetic differentiation, diversity, and drug susceptibility of Candida Krusei', Frontiers in Microbiology, vol. 9, no. NOV, 2717, pp. 1-10. https://doi.org/10.3389/fmicb.2018.02717

Genetic differentiation, diversity, and drug susceptibility of Candida Krusei. / Gong, Jie; Xiao, Meng; Wang, He; Kudinha, Timothy; Wang, Yu; Zhao, Fei; Wu, Weiwei; He, Lihua; Xu, Ying Chun; Zhang, Jianzhong.

In: Frontiers in Microbiology, Vol. 9, No. NOV, 2717, 20.11.2018, p. 1-10.

Research output: Contribution to journalArticle

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T1 - Genetic differentiation, diversity, and drug susceptibility of Candida Krusei

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AU - Xiao, Meng

AU - Wang, He

AU - Kudinha, Timothy

AU - Wang, Yu

AU - Zhao, Fei

AU - Wu, Weiwei

AU - He, Lihua

AU - Xu, Ying Chun

AU - Zhang, Jianzhong

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