Genetic diversity within a population of Microlaena stipoides, as revealed by AFLP markers

Meredith Mitchell, Benjamin Stodart, James Virgona

Research output: Contribution to journalArticle

4 Citations (Scopus)
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Abstract

Microlaena stipoides (Labill.) R.Br. (microlaena), a C3 perennial grass, is common within grazed native pastures in the high-rainfall zone (>550mm average annual rainfall) of south-eastern Australia. It has the ability to spread via seed production or vegetatively, using both rhizomes and stolons. This experiment aimed to determine how variable a microlaena population was within a single area, with the aim of determining whether microlaena relied on seed or vegetative spread to sustain populations. Leaf samples of microlaena were collected from 85 locations, sampling two transects, within a pasture at Chiltern, in north-eastern Victoria (36°12S, 146°35E). The genetic diversity among samples was analysed using amplified fragment length polymorphism (AFLP) markers. We obtained 1612 fragments, using 10 primers combinations. Polymorphism for the markers ranged from 47% to 65%. These results indicated that the populations of microlaena that exist within the pasture at Chiltern are likely to have undergone some degree of outcrossing (Fst=0.0219). It is likely that recruitment is occurring from sexual reproduction as well as via clonal spread within the microlaena population examined. This ability to use vegetative spread as well as both sexual and asexual reproduction may make populations of microlaena more resilient in the longer term.
Original languageEnglish
Pages (from-to)580-586
Number of pages7
JournalAustralian Journal of Botany
Volume62
Issue number7
DOIs
Publication statusPublished - Feb 2014

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Microlaena
Microlaena stipoides
amplified fragment length polymorphism
pasture
polymorphism
sexual reproduction
genetic variation
rainfall
asexual reproduction
outcrossing
rhizome
seed production
pastures
transect
grass
Victoria (Australia)
seed
seed productivity
rain
sampling

Cite this

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title = "Genetic diversity within a population of Microlaena stipoides, as revealed by AFLP markers",
abstract = "Microlaena stipoides (Labill.) R.Br. (microlaena), a C3 perennial grass, is common within grazed native pastures in the high-rainfall zone (>550mm average annual rainfall) of south-eastern Australia. It has the ability to spread via seed production or vegetatively, using both rhizomes and stolons. This experiment aimed to determine how variable a microlaena population was within a single area, with the aim of determining whether microlaena relied on seed or vegetative spread to sustain populations. Leaf samples of microlaena were collected from 85 locations, sampling two transects, within a pasture at Chiltern, in north-eastern Victoria (36°12S, 146°35E). The genetic diversity among samples was analysed using amplified fragment length polymorphism (AFLP) markers. We obtained 1612 fragments, using 10 primers combinations. Polymorphism for the markers ranged from 47{\%} to 65{\%}. These results indicated that the populations of microlaena that exist within the pasture at Chiltern are likely to have undergone some degree of outcrossing (Fst=0.0219). It is likely that recruitment is occurring from sexual reproduction as well as via clonal spread within the microlaena population examined. This ability to use vegetative spread as well as both sexual and asexual reproduction may make populations of microlaena more resilient in the longer term.",
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Genetic diversity within a population of Microlaena stipoides, as revealed by AFLP markers. / Mitchell, Meredith; Stodart, Benjamin; Virgona, James.

In: Australian Journal of Botany, Vol. 62, No. 7, 02.2014, p. 580-586.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic diversity within a population of Microlaena stipoides, as revealed by AFLP markers

AU - Mitchell, Meredith

AU - Stodart, Benjamin

AU - Virgona, James

N1 - Includes bibliographical references.

PY - 2014/2

Y1 - 2014/2

N2 - Microlaena stipoides (Labill.) R.Br. (microlaena), a C3 perennial grass, is common within grazed native pastures in the high-rainfall zone (>550mm average annual rainfall) of south-eastern Australia. It has the ability to spread via seed production or vegetatively, using both rhizomes and stolons. This experiment aimed to determine how variable a microlaena population was within a single area, with the aim of determining whether microlaena relied on seed or vegetative spread to sustain populations. Leaf samples of microlaena were collected from 85 locations, sampling two transects, within a pasture at Chiltern, in north-eastern Victoria (36°12S, 146°35E). The genetic diversity among samples was analysed using amplified fragment length polymorphism (AFLP) markers. We obtained 1612 fragments, using 10 primers combinations. Polymorphism for the markers ranged from 47% to 65%. These results indicated that the populations of microlaena that exist within the pasture at Chiltern are likely to have undergone some degree of outcrossing (Fst=0.0219). It is likely that recruitment is occurring from sexual reproduction as well as via clonal spread within the microlaena population examined. This ability to use vegetative spread as well as both sexual and asexual reproduction may make populations of microlaena more resilient in the longer term.

AB - Microlaena stipoides (Labill.) R.Br. (microlaena), a C3 perennial grass, is common within grazed native pastures in the high-rainfall zone (>550mm average annual rainfall) of south-eastern Australia. It has the ability to spread via seed production or vegetatively, using both rhizomes and stolons. This experiment aimed to determine how variable a microlaena population was within a single area, with the aim of determining whether microlaena relied on seed or vegetative spread to sustain populations. Leaf samples of microlaena were collected from 85 locations, sampling two transects, within a pasture at Chiltern, in north-eastern Victoria (36°12S, 146°35E). The genetic diversity among samples was analysed using amplified fragment length polymorphism (AFLP) markers. We obtained 1612 fragments, using 10 primers combinations. Polymorphism for the markers ranged from 47% to 65%. These results indicated that the populations of microlaena that exist within the pasture at Chiltern are likely to have undergone some degree of outcrossing (Fst=0.0219). It is likely that recruitment is occurring from sexual reproduction as well as via clonal spread within the microlaena population examined. This ability to use vegetative spread as well as both sexual and asexual reproduction may make populations of microlaena more resilient in the longer term.

KW - DNA

KW - Leaf samples

KW - Native grass

KW - Pasture

KW - Poaceae

KW - Variation

KW - Weeping grass

U2 - 10.1071/BT14182

DO - 10.1071/BT14182

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SP - 580

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JO - Australian Journal of Botany

JF - Australian Journal of Botany

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