TY - JOUR
T1 - Genetic variation in the OPN gene affects milk composition in Chinese Holstein cows
AU - Raza, Sayed
AU - Khan, Rajwali
AU - Pant, Sam
AU - Ali Shah, Mujahid
AU - Quan, Guobo
AU - Feng, Long
AU - Cheng, Gong
AU - Gui, Lin-sheng
AU - Zan, Linsen
N1 - Includes bibliographical references
PY - 2021/11/15
Y1 - 2021/11/15
N2 - The aim of this study was to investigate the association between genotypes and haplotypes of OPN, and milk composition in dairy cows. A total of 317 Chinese Holstein cows were genotyped via DNA sequencing in this study. Three single nucleotide polymorphisms (SNPs), g.2916G > A, g.58675C > T and g.58899C > A, and eight haplotypes were identified. Of the eight possible haplotypes, four haplotypes i.e., Hap2 (ACC; 55.30%), Hap6 (GCC, 15.6%), Hap1 (ACA, 13.6%) and Hap4 (ATC, 5.70%), were considered to be major with a cumulative estimated frequency of >90%. Single markers (g.2916G > A and g.58899C > A) and Haplotype Hap6/4 were found to be associated with an increase in butter-fat percentage (p < 0.05). Taken together, our results provided evidence that polymorphisms in OPN are associated with milk composition, and could potentially be used for marker-assisted selection in Chinese Holstein cows.
AB - The aim of this study was to investigate the association between genotypes and haplotypes of OPN, and milk composition in dairy cows. A total of 317 Chinese Holstein cows were genotyped via DNA sequencing in this study. Three single nucleotide polymorphisms (SNPs), g.2916G > A, g.58675C > T and g.58899C > A, and eight haplotypes were identified. Of the eight possible haplotypes, four haplotypes i.e., Hap2 (ACC; 55.30%), Hap6 (GCC, 15.6%), Hap1 (ACA, 13.6%) and Hap4 (ATC, 5.70%), were considered to be major with a cumulative estimated frequency of >90%. Single markers (g.2916G > A and g.58899C > A) and Haplotype Hap6/4 were found to be associated with an increase in butter-fat percentage (p < 0.05). Taken together, our results provided evidence that polymorphisms in OPN are associated with milk composition, and could potentially be used for marker-assisted selection in Chinese Holstein cows.
KW - genetic variability
KW - haplotype
KW - milk composition
KW - OPN gene
KW - single nucleotide polymorphism
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U2 - 10.1080/10495398.2021.2001343
DO - 10.1080/10495398.2021.2001343
M3 - Article
C2 - 34779705
SN - 1049-5398
VL - 34
SP - 893
EP - 899
JO - Animal Biotechnology
JF - Animal Biotechnology
IS - 4
ER -