Genetic variation in the OPN gene affects milk composition in Chinese Holstein cows

Sayed Raza, Rajwali Khan, Sam Pant, Mujahid Ali Shah, Guobo Quan, Long Feng, Gong Cheng, Lin-sheng Gui, Linsen Zan

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The aim of this study was to investigate the association between genotypes and haplotypes of OPN, and milk composition in dairy cows. A total of 317 Chinese Holstein cows were genotyped via DNA sequencing in this study. Three single nucleotide polymorphisms (SNPs), g.2916G > A, g.58675C > T and g.58899C > A, and eight haplotypes were identified. Of the eight possible haplotypes, four haplotypes i.e., Hap2 (ACC; 55.30%), Hap6 (GCC, 15.6%), Hap1 (ACA, 13.6%) and Hap4 (ATC, 5.70%), were considered to be major with a cumulative estimated frequency of >90%. Single markers (g.2916G > A and g.58899C > A) and Haplotype Hap6/4 were found to be associated with an increase in butter-fat percentage (p < 0.05). Taken together, our results provided evidence that polymorphisms in OPN are associated with milk composition, and could potentially be used for marker-assisted selection in Chinese Holstein cows.

Original languageEnglish
Pages (from-to)893-899
Number of pages7
JournalAnimal Biotechnology
Issue number4
Early online date15 Nov 2021
Publication statusPublished - Nov 2023


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