Genetic variation in the OPN gene affects milk composition in Chinese Holstein cows

Sayed Raza; Rajwali Khan; Sam Pant; Mujahid Ali Shah; Guobo Quan; Long  Feng; Gong Cheng; Lin-sheng Gui; Linsen Zan

doi:10.1080/10495398.2021.2001343

Genetic variation in the OPN gene affects milk composition in Chinese Holstein cows

Sayed Raza, Rajwali Khan, Sam Pant, Mujahid Ali Shah, Guobo Quan, Long Feng, Gong Cheng, Lin-sheng Gui, Linsen Zan

Agricultural, Environmental and Veterinary Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to investigate the association between genotypes and haplotypes of OPN,and milk composition in dairy cows. A total of 317 Chinese Holstein cows were genotyped via DNA sequencing in this study. Three single nucleotide polymorphisms (SNPs), g.2916G>A, g.58675C>T and g.58899C>A, and eight haplotypes were identified. Of theeight possible haplotypes, four haplotypes i.e., Hap2 (ACC; 55.30%), Hap6 (GCC, 15.6%), Hap1 (ACA, 13.6%) and Hap4 (ATC, 5.70%), were considered to be major with a cumulative estimated frequency of>90%. Single markers (g.2916G>A and g.58899C>A) and Haplotype Hap6/4 were found to be associated with an increase in butter-fat percentage(p<0.05). Taken together, our results provided evidence that polymorphisms in OPN are associated with milk composition, and could potentially be used for marker-assisted selection in Chinese Holstein cows.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Animal Biotechnology
DOIs	https://doi.org/10.1080/10495398.2021.2001343
Publication status	E-pub ahead of print - 15 Nov 2021

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@article{b08747637bf141bd9d3c20773b976cdd,

title = "Genetic variation in the OPN gene affects milk composition in Chinese Holstein cows",

abstract = "The aim of this study was to investigate the association between genotypes and haplotypes of OPN,and milk composition in dairy cows. A total of 317 Chinese Holstein cows were genotyped via DNA sequencing in this study. Three single nucleotide polymorphisms (SNPs), g.2916G>A, g.58675C>T and g.58899C>A, and eight haplotypes were identified. Of theeight possible haplotypes, four haplotypes i.e., Hap2 (ACC; 55.30%), Hap6 (GCC, 15.6%), Hap1 (ACA, 13.6%) and Hap4 (ATC, 5.70%), were considered to be major with a cumulative estimated frequency of>90%. Single markers (g.2916G>A and g.58899C>A) and Haplotype Hap6/4 were found to be associated with an increase in butter-fat percentage(p<0.05). Taken together, our results provided evidence that polymorphisms in OPN are associated with milk composition, and could potentially be used for marker-assisted selection in Chinese Holstein cows.",

author = "Sayed Raza and Rajwali Khan and Sam Pant and {Ali Shah}, Mujahid and Guobo Quan and Long Feng and Gong Cheng and Lin-sheng Gui and Linsen Zan",

year = "2021",

month = nov,

day = "15",

doi = "10.1080/10495398.2021.2001343",

language = "English",

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journal = "Animal Biotechnology",

issn = "1049-5398",

publisher = "Taylor & Francis",

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T1 - Genetic variation in the OPN gene affects milk composition in Chinese Holstein cows

AU - Raza, Sayed

AU - Khan, Rajwali

AU - Pant, Sam

AU - Ali Shah, Mujahid

AU - Quan, Guobo

AU - Feng, Long

AU - Cheng, Gong

AU - Gui, Lin-sheng

AU - Zan, Linsen

PY - 2021/11/15

Y1 - 2021/11/15

N2 - The aim of this study was to investigate the association between genotypes and haplotypes of OPN,and milk composition in dairy cows. A total of 317 Chinese Holstein cows were genotyped via DNA sequencing in this study. Three single nucleotide polymorphisms (SNPs), g.2916G>A, g.58675C>T and g.58899C>A, and eight haplotypes were identified. Of theeight possible haplotypes, four haplotypes i.e., Hap2 (ACC; 55.30%), Hap6 (GCC, 15.6%), Hap1 (ACA, 13.6%) and Hap4 (ATC, 5.70%), were considered to be major with a cumulative estimated frequency of>90%. Single markers (g.2916G>A and g.58899C>A) and Haplotype Hap6/4 were found to be associated with an increase in butter-fat percentage(p<0.05). Taken together, our results provided evidence that polymorphisms in OPN are associated with milk composition, and could potentially be used for marker-assisted selection in Chinese Holstein cows.

AB - The aim of this study was to investigate the association between genotypes and haplotypes of OPN,and milk composition in dairy cows. A total of 317 Chinese Holstein cows were genotyped via DNA sequencing in this study. Three single nucleotide polymorphisms (SNPs), g.2916G>A, g.58675C>T and g.58899C>A, and eight haplotypes were identified. Of theeight possible haplotypes, four haplotypes i.e., Hap2 (ACC; 55.30%), Hap6 (GCC, 15.6%), Hap1 (ACA, 13.6%) and Hap4 (ATC, 5.70%), were considered to be major with a cumulative estimated frequency of>90%. Single markers (g.2916G>A and g.58899C>A) and Haplotype Hap6/4 were found to be associated with an increase in butter-fat percentage(p<0.05). Taken together, our results provided evidence that polymorphisms in OPN are associated with milk composition, and could potentially be used for marker-assisted selection in Chinese Holstein cows.

U2 - 10.1080/10495398.2021.2001343

DO - 10.1080/10495398.2021.2001343

M3 - Article

C2 - 34779705

SP - 1

EP - 7

JO - Animal Biotechnology

JF - Animal Biotechnology

SN - 1049-5398

ER -

Genetic variation in the OPN gene affects milk composition in Chinese Holstein cows

Abstract

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