Genome sequence of an Australian strain of canid alphaherpesvirus 1

Subir Sarker, S. Das, K. Helbig, A. Peters, S. R. Raidal

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective
Characterisation of a complete genome sequence of an Australian strain of canid alphaherpesvirus 1 (CHV-1) and its phylogenetic relationship with other varicellovirus species.

Methods
Standard pathology and PCR methods were used to initially detect herpesvirus in hepatic tissue from an infected 4-week-old Labrador Retriever puppy. The complete CHV-1 genome was sequenced using next-generation sequencing technology followed by de novo and reference assembly, and genome annotation.

Results
The CHV-1 genome was 125 kbp in length and contained 74 predicted open reading frames encoding functional proteins, all of which have counterparts in other alphaherpesviruses. Phylogenetic analysis using the DNA polymerase gene revealed that the newly sequenced CHV-1 clustered with canid alphaherpesvirus isolated from the UK and shared a 99% overall nucleotide sequence similarity.

Conclusion
This is the first complete genome of an Australian strain of CHV-1, which will contribute to our understanding of the genetics and evolution of herpesvirus.
Original languageEnglish
Pages (from-to)24-27
Number of pages4
JournalAustralian Veterinary Journal
Volume96
Issue number1-2
Early online date19 Dec 2017
DOIs
Publication statusPublished - 01 Jan 2018

Fingerprint

Canidae
Genome
genome
Herpesviridae
Varicellovirus
Newfoundland and Labrador
Molecular Evolution
DNA-Directed DNA Polymerase
Labrador Retriever
phylogeny
puppies
DNA-directed DNA polymerase
Open Reading Frames
open reading frames
Pathology
Technology
Polymerase Chain Reaction
Liver
nucleotide sequences
liver

Cite this

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abstract = "ObjectiveCharacterisation of a complete genome sequence of an Australian strain of canid alphaherpesvirus 1 (CHV-1) and its phylogenetic relationship with other varicellovirus species.MethodsStandard pathology and PCR methods were used to initially detect herpesvirus in hepatic tissue from an infected 4-week-old Labrador Retriever puppy. The complete CHV-1 genome was sequenced using next-generation sequencing technology followed by de novo and reference assembly, and genome annotation.ResultsThe CHV-1 genome was 125 kbp in length and contained 74 predicted open reading frames encoding functional proteins, all of which have counterparts in other alphaherpesviruses. Phylogenetic analysis using the DNA polymerase gene revealed that the newly sequenced CHV-1 clustered with canid alphaherpesvirus isolated from the UK and shared a 99{\%} overall nucleotide sequence similarity.ConclusionThis is the first complete genome of an Australian strain of CHV-1, which will contribute to our understanding of the genetics and evolution of herpesvirus.",
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Genome sequence of an Australian strain of canid alphaherpesvirus 1. / Sarker, Subir; Das, S.; Helbig, K.; Peters, A.; Raidal, S. R.

In: Australian Veterinary Journal, Vol. 96, No. 1-2, 01.01.2018, p. 24-27.

Research output: Contribution to journalArticle

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AU - Das, S.

AU - Helbig, K.

AU - Peters, A.

AU - Raidal, S. R.

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Y1 - 2018/1/1

N2 - ObjectiveCharacterisation of a complete genome sequence of an Australian strain of canid alphaherpesvirus 1 (CHV-1) and its phylogenetic relationship with other varicellovirus species.MethodsStandard pathology and PCR methods were used to initially detect herpesvirus in hepatic tissue from an infected 4-week-old Labrador Retriever puppy. The complete CHV-1 genome was sequenced using next-generation sequencing technology followed by de novo and reference assembly, and genome annotation.ResultsThe CHV-1 genome was 125 kbp in length and contained 74 predicted open reading frames encoding functional proteins, all of which have counterparts in other alphaherpesviruses. Phylogenetic analysis using the DNA polymerase gene revealed that the newly sequenced CHV-1 clustered with canid alphaherpesvirus isolated from the UK and shared a 99% overall nucleotide sequence similarity.ConclusionThis is the first complete genome of an Australian strain of CHV-1, which will contribute to our understanding of the genetics and evolution of herpesvirus.

AB - ObjectiveCharacterisation of a complete genome sequence of an Australian strain of canid alphaherpesvirus 1 (CHV-1) and its phylogenetic relationship with other varicellovirus species.MethodsStandard pathology and PCR methods were used to initially detect herpesvirus in hepatic tissue from an infected 4-week-old Labrador Retriever puppy. The complete CHV-1 genome was sequenced using next-generation sequencing technology followed by de novo and reference assembly, and genome annotation.ResultsThe CHV-1 genome was 125 kbp in length and contained 74 predicted open reading frames encoding functional proteins, all of which have counterparts in other alphaherpesviruses. Phylogenetic analysis using the DNA polymerase gene revealed that the newly sequenced CHV-1 clustered with canid alphaherpesvirus isolated from the UK and shared a 99% overall nucleotide sequence similarity.ConclusionThis is the first complete genome of an Australian strain of CHV-1, which will contribute to our understanding of the genetics and evolution of herpesvirus.

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