Genome walking

Frances M. Shapter, Daniel L E Waters

Research output: Book chapter/Published conference paperChapter in textbook/reference book

4 Citations (Scopus)

Abstract

Genome walking is a method for determining the DNA sequence of unknown genomic regions flanking a region of known DNA sequence. The Genome walking has the potential to capture 6-7 kb of sequence in a single round. Ideal for identifying gene promoter regions where only the coding region. Genome walking also has significant utility for capturing homologous genes in new species when there are areas in the target gene with strong sequence conservation to the characterized species. The increasing use of next-generation sequencing technologies will see the principles of genome walking adapted to in silico methods. However, for smaller projects, PCR-based genome walking will remain an efficient method of characterizing unknown flanking sequence.
Original languageEnglish
Title of host publicationCereal Genomics
Subtitle of host publicationMethods and Protocols
EditorsR. Henry, A. Furtado
Place of PublicationTotowa, NJ
PublisherHumana Press Inc.
Chapter12
Pages133-146
Number of pages14
Volume1099
ISBN (Electronic)9781627037150
ISBN (Print)9781627037143
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1099
ISSN (Print)1064-3745

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