TY - JOUR
T1 - Graphene-oxide-loaded superparamagnetic iron oxide nanoparticles for ultrasensitive electrocatalytic detection of microRNA
AU - Islam, Md Nazmul
AU - Gorgannezhad, Lena
AU - Masud, Mostafa Kamal
AU - Tanaka, Shunsuke
AU - Hossain, Md Shahriar A.
AU - Yamauchi, Yusuke
AU - Nguyen, Nam Trung
AU - Shiddiky, Muhammad J.A.
N1 - Publisher Copyright:
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/9/3
Y1 - 2018/9/3
N2 - We report the electrocatalytic activity of a new class of superparamagnetic nanoparticles, graphene-oxide-loaded iron oxide (GO/IO hybrid material), towards the reduction of ruthenium hexaammine(III) chloride (Ru(NH3)6]3+, RuHex). Leveraging the electrocatalytic activity of the GO/IO hybrid material and the signal enhancement capacity of [Ru(NH3)6]3+/[Fe(CN)6]3− in an electrocatalytic cycle, an ultrasensitive and specific electrochemical sensor was developed for the detection of cancer-related microRNA (miRNA). Using the direct affinity interaction between RNA and graphene oxide, magnetically isolated and purified target miRNA were directly adsorbed onto a screen-printed electrode modified with the GO/IO hybrid material. The detection was enabled by chronocoulometric (CC) readout of charge-compensating [Ru(NH3)6]3+ followed by an enhancement in CC charge display through the Ru(NH3)6]3+/[Fe(CN)6]3− system. We demonstrate an excellent limit of detection of 1.0 fM by accurately detecting miR-21 in synthetic samples and showcase its clinical utility in ovarian cancer cell lines with high sensitivity (ten cells) and good reproducibility (% RSD=<5 %, for n=3).
AB - We report the electrocatalytic activity of a new class of superparamagnetic nanoparticles, graphene-oxide-loaded iron oxide (GO/IO hybrid material), towards the reduction of ruthenium hexaammine(III) chloride (Ru(NH3)6]3+, RuHex). Leveraging the electrocatalytic activity of the GO/IO hybrid material and the signal enhancement capacity of [Ru(NH3)6]3+/[Fe(CN)6]3− in an electrocatalytic cycle, an ultrasensitive and specific electrochemical sensor was developed for the detection of cancer-related microRNA (miRNA). Using the direct affinity interaction between RNA and graphene oxide, magnetically isolated and purified target miRNA were directly adsorbed onto a screen-printed electrode modified with the GO/IO hybrid material. The detection was enabled by chronocoulometric (CC) readout of charge-compensating [Ru(NH3)6]3+ followed by an enhancement in CC charge display through the Ru(NH3)6]3+/[Fe(CN)6]3− system. We demonstrate an excellent limit of detection of 1.0 fM by accurately detecting miR-21 in synthetic samples and showcase its clinical utility in ovarian cancer cell lines with high sensitivity (ten cells) and good reproducibility (% RSD=<5 %, for n=3).
KW - electrocatalysis
KW - electrochemical detection
KW - graphene oxide/iron oxide hybrid
KW - microRNA detection
KW - ovarian cancer
KW - screen-printed electrodes
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UR - http://www.scopus.com/inward/citedby.url?scp=85052739291&partnerID=8YFLogxK
U2 - 10.1002/celc.201800339
DO - 10.1002/celc.201800339
M3 - Article
AN - SCOPUS:85052739291
SN - 2196-0216
VL - 5
SP - 2488
EP - 2495
JO - ChemElectroChem
JF - ChemElectroChem
IS - 17
ER -
