Hepatitis B virus surface antigen variants clustered within immune epitopes in chronic hepatitis B carriers from Hormozgan province, south of Iran

Mehdi Norouzi, Seyed Ali Ghorashi, Behrooz Ataei, Majid Yaran, Reza Malekzadeh, Seyed Moayyed Alavian, Mohammad Ali Judaki, Shiva Ghamari, Alireza Namazi, Ramin Rahimnia, Abolfazl Khedive, Seyed Mohammad Jazayeri

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective(s): The aim of this study was to characterize the hepatitis B virus surface protein genotypes and sequence variations among hepatitis B virus surface antigen (HBsAg) positive chronic patients in Hormozgan province, south of Iran. Materials and Methods: A total of 8 patients enrolled in this study. The surface gene was amplified and directly sequenced. Genotypes and nucleotide/amino acid substitutions were identified compared to the sequences obtained from the database. Results: All strains belonged to genotype D. Overall 77 "mutations" occurred at 45 nucleotide positions, of them, 44 (57.14%) were silent (no amino acid altering) and 33 (42.86%) were missense (amino acid changing). A number of 24 (80%) out of 30 amino acid changes occurred in different immune epitopes within surface protein, of which, 9 (30%) in B cell epitopes in 7 residues (2 occurred in "a" determinant region); 8 (42.1%) in T helper epitopes in 7 residues and 7 (10%) in 4 residues inside CTL epitopes. Conclusion: Hepatitis B virus genome containing mutated immune epitopes no longer could be recognized by specific T-cells of the host immune surveillance and did not enhance anti-HBs production. This could led to the progression of chronicity of hepatitis B virus infection.

Original languageEnglish
Pages (from-to)213-224
Number of pages12
JournalIranian Journal of Basic Medical Sciences
Volume13
Issue number4
Publication statusPublished - 01 Sep 2010

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