High throughput analysis of red wine and grape phenolics adaptation and validation of methyl cellulose precipitable tannin assay and modified somers color assay to a rapid 96 well plate format

Meagan D. Mercurio, Robert Dambergs, Markus Herderich, Paul A Smith

Research output: Contribution to journalArticlepeer-review

178 Citations (Scopus)

Abstract

The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.
Keywords: Tannins; anthocyanins; quantification; grape; red wine; wine color; assay; methyl cellulose precipitation
Original languageEnglish
Pages (from-to)4651-4657
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number12
DOIs
Publication statusPublished - 12 May 2007

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