Human papillomavirus infection in non-noplastic uterine cervical disease in Hong Kong

C Lee, Heather Cavanagh, S Lo, C Ng

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The polymerase chain reaction (PCR) was used to detect and identify human papillomavirus (HPV) in 108 cases of formalin-fixed, paraffin-embedded, non-neoplastic uterine cervical biopsy tissue retrieved from the surgical pathology archives of the Department of Pathology, Caritas Medical Centre, Hong Kong. After DNA extraction, HPV L1 gene primers were used to detect the presence of HPV, and type-specific primers (to HPV types 6, 11, 16, 18, 31 and 33) were used to identify the specific HPV type on HPV L1-positive cases. PCR amplification of the beta-globin gene was used to ensure the quality of amplifiable DNA extracted. Of 94 cases that yielded sufficient good-quality DNA for PCR analysis, three (one endocervical polyp, one chronic inflammation with erosion, and a normal biopsy) had detectable HPV infection. Two of these had high-risk HPV type 16; the other had an uncommon HPV type. In view of the low incidence of HPV found in these patients, large-scale population screening of clinical samples using PCR to detect the presence of HPV and identify high-risk asymptomatic patients would not be cost-effective.
Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalBritish Journal of Biomedical Science
Volume58
Issue number2
Publication statusPublished - 2001

Fingerprint

Uterine Cervical Diseases
Papillomavirus Infections
Polymerase chain reaction
Hong Kong
Biopsy
Pathology
Genes
beta-Globins
DNA
Polymerase Chain Reaction
DNA-Directed DNA Polymerase
Paraffin
Formaldehyde
Amplification
Erosion
Screening
Tissue
Human papillomavirus 11
Human papillomavirus 6
Surgical Pathology

Cite this

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abstract = "The polymerase chain reaction (PCR) was used to detect and identify human papillomavirus (HPV) in 108 cases of formalin-fixed, paraffin-embedded, non-neoplastic uterine cervical biopsy tissue retrieved from the surgical pathology archives of the Department of Pathology, Caritas Medical Centre, Hong Kong. After DNA extraction, HPV L1 gene primers were used to detect the presence of HPV, and type-specific primers (to HPV types 6, 11, 16, 18, 31 and 33) were used to identify the specific HPV type on HPV L1-positive cases. PCR amplification of the beta-globin gene was used to ensure the quality of amplifiable DNA extracted. Of 94 cases that yielded sufficient good-quality DNA for PCR analysis, three (one endocervical polyp, one chronic inflammation with erosion, and a normal biopsy) had detectable HPV infection. Two of these had high-risk HPV type 16; the other had an uncommon HPV type. In view of the low incidence of HPV found in these patients, large-scale population screening of clinical samples using PCR to detect the presence of HPV and identify high-risk asymptomatic patients would not be cost-effective.",
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Human papillomavirus infection in non-noplastic uterine cervical disease in Hong Kong. / Lee, C; Cavanagh, Heather; Lo, S; Ng, C.

In: British Journal of Biomedical Science, Vol. 58, No. 2, 2001, p. 85-91.

Research output: Contribution to journalArticle

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