Hypoxia in grape berries

The role of seed respiration and lenticels on the berry pedicel and the possible link to cell death

Zeyu Xiao, Suzy Y. Rogiers, Victor O. Sadras, Stephen D. Tyerman

Research output: Contribution to journalArticle

6 Citations (Scopus)
8 Downloads (Pure)

Abstract

Mesocarp cell death (CD) during ripening is common in berries of seeded Vitis vinifera L. wine cultivars. We examined if hypoxia within berries is linked to CD. The internal oxygen concentration ([O 2 ]) across the mesocarp was measured in berries from Chardonnay and Shiraz, both seeded, and Ruby Seedless, using an oxygen micro-sensor. Steep [O 2 ] gradients were observed across the skin and [O 2 ] decreased toward the middle of the mesocarp. As ripening progressed, the minimum [O 2 ] approached zero in the seeded cultivars and correlated to the profile of CD across the mesocarp. Seed respiration declined during ripening, from a large proportion of total berry respiration early to negligible at later stages. [O 2 ] increased towards the central axis corresponding to the presence of air spaces visualized using X-ray micro-computed tomography (CT). These air spaces connect to the pedicel where lenticels are located that are critical for berry O 2 uptake as a function of temperature, and when blocked caused hypoxia in Chardonnay berries, ethanol accumulation, and CD. The implications of hypoxia in grape berries are discussed in terms of its role in CD, ripening, and berry water relations.

Original languageEnglish
Pages (from-to)2071-2083
Number of pages13
JournalJournal of Experimental Botany
Volume69
Issue number8
Early online date06 Mar 2018
DOIs
Publication statusPublished - 09 Apr 2018

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pedicel
Vitis
small fruits
cell death
hypoxia
grapes
Fruit
Seeds
Respiration
Cell Death
mesocarp
seeds
ripening
wine cultivars
oxygen
Air
micro-computed tomography
air
Oxygen
X-Ray Microtomography

Cite this

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title = "Hypoxia in grape berries: The role of seed respiration and lenticels on the berry pedicel and the possible link to cell death",
abstract = "Mesocarp cell death (CD) during ripening is common in berries of seeded Vitis vinifera L. wine cultivars. We examined if hypoxia within berries is linked to CD. The internal oxygen concentration ([O 2 ]) across the mesocarp was measured in berries from Chardonnay and Shiraz, both seeded, and Ruby Seedless, using an oxygen micro-sensor. Steep [O 2 ] gradients were observed across the skin and [O 2 ] decreased toward the middle of the mesocarp. As ripening progressed, the minimum [O 2 ] approached zero in the seeded cultivars and correlated to the profile of CD across the mesocarp. Seed respiration declined during ripening, from a large proportion of total berry respiration early to negligible at later stages. [O 2 ] increased towards the central axis corresponding to the presence of air spaces visualized using X-ray micro-computed tomography (CT). These air spaces connect to the pedicel where lenticels are located that are critical for berry O 2 uptake as a function of temperature, and when blocked caused hypoxia in Chardonnay berries, ethanol accumulation, and CD. The implications of hypoxia in grape berries are discussed in terms of its role in CD, ripening, and berry water relations.",
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Hypoxia in grape berries : The role of seed respiration and lenticels on the berry pedicel and the possible link to cell death. / Xiao, Zeyu; Rogiers, Suzy Y.; Sadras, Victor O.; Tyerman, Stephen D.

In: Journal of Experimental Botany, Vol. 69, No. 8, 09.04.2018, p. 2071-2083.

Research output: Contribution to journalArticle

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T2 - The role of seed respiration and lenticels on the berry pedicel and the possible link to cell death

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