Identification and characterization of a novel transcript down-regulation in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

Andrea Faedo, Jane C. Quinn, Patrick Stoney, Jason E. Long, Catherine Dye, Massimo Zollo, John L.R. Rubenstein, David J. Price, Alessandro Bulfone

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/D1x2-/- subpallium and Pax6 -/- pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2 -/- subpallium and up-regulated in the Pax6-/- pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2-/- and Pax6-/- mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2-/- subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6-/- lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.
Original languageEnglish
Pages (from-to)614-620
Number of pages7
JournalDevelopmental Dynamics
Volume231
Issue number3
DOIs
Publication statusPublished - Nov 2004

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Telencephalon
Down-Regulation
Median Eminence
Prosencephalon
Brain
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Thalamus
Transcriptome
Hypothalamus
In Situ Hybridization
RNA

Cite this

Faedo, Andrea ; Quinn, Jane C. ; Stoney, Patrick ; Long, Jason E. ; Dye, Catherine ; Zollo, Massimo ; Rubenstein, John L.R. ; Price, David J. ; Bulfone, Alessandro. / Identification and characterization of a novel transcript down-regulation in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon. In: Developmental Dynamics. 2004 ; Vol. 231, No. 3. pp. 614-620.
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Identification and characterization of a novel transcript down-regulation in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon. / Faedo, Andrea; Quinn, Jane C.; Stoney, Patrick; Long, Jason E.; Dye, Catherine; Zollo, Massimo; Rubenstein, John L.R.; Price, David J.; Bulfone, Alessandro.

In: Developmental Dynamics, Vol. 231, No. 3, 11.2004, p. 614-620.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification and characterization of a novel transcript down-regulation in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

AU - Faedo, Andrea

AU - Quinn, Jane C.

AU - Stoney, Patrick

AU - Long, Jason E.

AU - Dye, Catherine

AU - Zollo, Massimo

AU - Rubenstein, John L.R.

AU - Price, David J.

AU - Bulfone, Alessandro

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = November, 2004; Journal title (773t) = Developmental Dynamics. ISSNs: 1058-8388;

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N2 - By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/D1x2-/- subpallium and Pax6 -/- pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2 -/- subpallium and up-regulated in the Pax6-/- pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2-/- and Pax6-/- mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2-/- subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6-/- lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

AB - By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/D1x2-/- subpallium and Pax6 -/- pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2 -/- subpallium and up-regulated in the Pax6-/- pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2-/- and Pax6-/- mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2-/- subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6-/- lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

KW - Dlx1-Dlx2

KW - Dlx5-Dlx6

KW - Dorsoventral patterning

KW - Evf-1

KW - Microarray

KW - Mouse

KW - Pax6

KW - Telencephalic development

U2 - 10.1002/dvdy.20152

DO - 10.1002/dvdy.20152

M3 - Article

VL - 231

SP - 614

EP - 620

JO - American Journal of Anatomy

JF - American Journal of Anatomy

SN - 1058-8388

IS - 3

ER -