Identification and expression profiling of putative chemosensory protein genes in two rice planthoppers, Laodelphax striatellus (Fallén) and Sogatella furcifera (Horváth)

Wen-Wu Zhou, Xin Yuan, Ping Qian, Jiaan Cheng, Chuanxi Zhang, Geoffrey Gurr, Zengrong Zhu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chemosensory proteins (CSP) afford insects the ability to recognize complicated environmental cues and are involved in diverse physiological processes. The planthoppers Laodelphax striatellus and Sogatella furcifera are serious specialist pests of rice throughout eastern Asia. In this study, we identified 21 putative CSP genes (namely LsCSP1–12 and SfCSP1–9) in the two planthoppers L. striatellus and S. furcifera, and profiled their transcript accumulation in different developmental stages and body parts by real-time quantitative PCR (qPCR). All CSPs had the characteristic typical features of the CSP family, with the exception of LsCSP10 and SfCSP2, which lacked the predicted signal peptide sequence. Among these putative CSP genes, only two (LsCSP9 and SfCSP5) were more highly expressed in the antennae, indicating that they might be involved in olfaction. On the contrary, eleven genes (LsCSP2, 3, 4, 6, 8, 12; SfCSP1, 4, 6, 7, 8) and one gene (SfCSP9) were more highly expressed in the legs and heads (without antennae), respectively. The remainders of the genes were ubiquitously expressed in the heads (without antennae) and the bodies (without the legs and heads). The broad expression patterns in different body parts indicate that the CSPs of these two delphacid species may be involved in diverse functions besides chemosensation. These findings increase the understanding of the chemosensory protein system of Hemiptera species.
Original languageEnglish
Pages (from-to)771-778
Number of pages8
JournalJournal of Asia-Pacific Entomology
Volume18
Issue number4
DOIs
Publication statusPublished - 2015

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Laodelphax striatellus
Sogatella furcifera
Fulgoroidea
rice
antennae
genes
proteins
legs
insect proteins
smell
signal peptide
East Asia
Hemiptera
quantitative polymerase chain reaction
developmental stages
pests

Cite this

@article{5990c2e0460a4613855975c30153e562,
title = "Identification and expression profiling of putative chemosensory protein genes in two rice planthoppers, Laodelphax striatellus (Fall{\'e}n) and Sogatella furcifera (Horv{\'a}th)",
abstract = "Chemosensory proteins (CSP) afford insects the ability to recognize complicated environmental cues and are involved in diverse physiological processes. The planthoppers Laodelphax striatellus and Sogatella furcifera are serious specialist pests of rice throughout eastern Asia. In this study, we identified 21 putative CSP genes (namely LsCSP1–12 and SfCSP1–9) in the two planthoppers L. striatellus and S. furcifera, and profiled their transcript accumulation in different developmental stages and body parts by real-time quantitative PCR (qPCR). All CSPs had the characteristic typical features of the CSP family, with the exception of LsCSP10 and SfCSP2, which lacked the predicted signal peptide sequence. Among these putative CSP genes, only two (LsCSP9 and SfCSP5) were more highly expressed in the antennae, indicating that they might be involved in olfaction. On the contrary, eleven genes (LsCSP2, 3, 4, 6, 8, 12; SfCSP1, 4, 6, 7, 8) and one gene (SfCSP9) were more highly expressed in the legs and heads (without antennae), respectively. The remainders of the genes were ubiquitously expressed in the heads (without antennae) and the bodies (without the legs and heads). The broad expression patterns in different body parts indicate that the CSPs of these two delphacid species may be involved in diverse functions besides chemosensation. These findings increase the understanding of the chemosensory protein system of Hemiptera species.",
keywords = "Antennae, CSP, Delphacidae, Olfaction, Planthopper",
author = "Wen-Wu Zhou and Xin Yuan and Ping Qian and Jiaan Cheng and Chuanxi Zhang and Geoffrey Gurr and Zengrong Zhu",
note = "Includes bibliographical references.",
year = "2015",
doi = "10.1016/j.aspen.2015.09.006",
language = "English",
volume = "18",
pages = "771--778",
journal = "Journal of Asia-Pacific Entomology",
issn = "1226-8615",
publisher = "Elsevier",
number = "4",

}

Identification and expression profiling of putative chemosensory protein genes in two rice planthoppers, Laodelphax striatellus (Fallén) and Sogatella furcifera (Horváth). / Zhou, Wen-Wu; Yuan, Xin; Qian, Ping; Cheng, Jiaan; Zhang, Chuanxi; Gurr, Geoffrey; Zhu, Zengrong.

In: Journal of Asia-Pacific Entomology, Vol. 18, No. 4, 2015, p. 771-778.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification and expression profiling of putative chemosensory protein genes in two rice planthoppers, Laodelphax striatellus (Fallén) and Sogatella furcifera (Horváth)

AU - Zhou, Wen-Wu

AU - Yuan, Xin

AU - Qian, Ping

AU - Cheng, Jiaan

AU - Zhang, Chuanxi

AU - Gurr, Geoffrey

AU - Zhu, Zengrong

N1 - Includes bibliographical references.

PY - 2015

Y1 - 2015

N2 - Chemosensory proteins (CSP) afford insects the ability to recognize complicated environmental cues and are involved in diverse physiological processes. The planthoppers Laodelphax striatellus and Sogatella furcifera are serious specialist pests of rice throughout eastern Asia. In this study, we identified 21 putative CSP genes (namely LsCSP1–12 and SfCSP1–9) in the two planthoppers L. striatellus and S. furcifera, and profiled their transcript accumulation in different developmental stages and body parts by real-time quantitative PCR (qPCR). All CSPs had the characteristic typical features of the CSP family, with the exception of LsCSP10 and SfCSP2, which lacked the predicted signal peptide sequence. Among these putative CSP genes, only two (LsCSP9 and SfCSP5) were more highly expressed in the antennae, indicating that they might be involved in olfaction. On the contrary, eleven genes (LsCSP2, 3, 4, 6, 8, 12; SfCSP1, 4, 6, 7, 8) and one gene (SfCSP9) were more highly expressed in the legs and heads (without antennae), respectively. The remainders of the genes were ubiquitously expressed in the heads (without antennae) and the bodies (without the legs and heads). The broad expression patterns in different body parts indicate that the CSPs of these two delphacid species may be involved in diverse functions besides chemosensation. These findings increase the understanding of the chemosensory protein system of Hemiptera species.

AB - Chemosensory proteins (CSP) afford insects the ability to recognize complicated environmental cues and are involved in diverse physiological processes. The planthoppers Laodelphax striatellus and Sogatella furcifera are serious specialist pests of rice throughout eastern Asia. In this study, we identified 21 putative CSP genes (namely LsCSP1–12 and SfCSP1–9) in the two planthoppers L. striatellus and S. furcifera, and profiled their transcript accumulation in different developmental stages and body parts by real-time quantitative PCR (qPCR). All CSPs had the characteristic typical features of the CSP family, with the exception of LsCSP10 and SfCSP2, which lacked the predicted signal peptide sequence. Among these putative CSP genes, only two (LsCSP9 and SfCSP5) were more highly expressed in the antennae, indicating that they might be involved in olfaction. On the contrary, eleven genes (LsCSP2, 3, 4, 6, 8, 12; SfCSP1, 4, 6, 7, 8) and one gene (SfCSP9) were more highly expressed in the legs and heads (without antennae), respectively. The remainders of the genes were ubiquitously expressed in the heads (without antennae) and the bodies (without the legs and heads). The broad expression patterns in different body parts indicate that the CSPs of these two delphacid species may be involved in diverse functions besides chemosensation. These findings increase the understanding of the chemosensory protein system of Hemiptera species.

KW - Antennae

KW - CSP

KW - Delphacidae

KW - Olfaction

KW - Planthopper

U2 - 10.1016/j.aspen.2015.09.006

DO - 10.1016/j.aspen.2015.09.006

M3 - Article

VL - 18

SP - 771

EP - 778

JO - Journal of Asia-Pacific Entomology

JF - Journal of Asia-Pacific Entomology

SN - 1226-8615

IS - 4

ER -