Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm

Kyle B. Reynolds, Darren P. Cullerne, Anna El Tahchy, Vivien Rolland, Christopher L. Blanchard, Craig C. Wood, Surinder P. Singh, James R. Petrie

Research output: Contribution to journalArticle

Abstract

Cocos nucifera (coconut), a member of the Arecaceae family, is an economically important woody palm that is widely grown in tropical and subtropical regions. The coconut palm is well known for its ability to accumulate large amounts of oil, approximately 63% of the seed weight. Coconut oil varies significantly from other vegetable oils as it contains a high proportion of medium-chain fatty acids (MCFA; 85%). The unique composition of coconut oil raises interest in understanding how the coconut palm produces oil of a high saturated MCFA content, and if such an oil profile could be replicated via biotechnology interventions. Although some gene discovery work has been performed there is still a significant gap in the knowledge associated with coconut's oil production pathways. In this study, a de novo transcriptome was assembled for developing coconut endosperm to identify genes involved in the synthesis of lipids, particularly triacylglycerol. Of particular interest were thioesterases, acyltransferases and oleosins because of their involvement in the processes of releasing fatty acids for assembly, esterification of fatty acids into glycerolipids and protecting oils from degradation, respectively. It is hypothesized that some of these genes may exhibit a strong substrate preference for MCFA and hence may assist the future development of vegetable oils with an enriched MCFA composition. In this study, we identified and confirmed functionality of five candidate genes from the gene families of interest. This study will benefit future work in areas of increasing vegetable oil production and the tailoring of oil fatty acid compositions.

Original languageEnglish
Pages (from-to)945-960
Number of pages16
JournalPlant & cell physiology
Volume60
Issue number5
DOIs
Publication statusPublished - 01 May 2019

Fingerprint

Cocos
Endosperm
Cocos nucifera
Transcriptome
transcriptome
endosperm
biosynthesis
Plant Oils
coconut oil
Lipids
Oils
Fatty Acids
oils
vegetable oil
lipids
Genes
coconuts
genes
Arecaceae
oleosin

Cite this

Reynolds, Kyle B. ; Cullerne, Darren P. ; El Tahchy, Anna ; Rolland, Vivien ; Blanchard, Christopher L. ; Wood, Craig C. ; Singh, Surinder P. ; Petrie, James R. / Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm. In: Plant & cell physiology. 2019 ; Vol. 60, No. 5. pp. 945-960.
@article{4bfebb653b464f6c89f637a20a759bca,
title = "Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm",
abstract = "Cocos nucifera (coconut), a member of the Arecaceae family, is an economically important woody palm that is widely grown in tropical and subtropical regions. The coconut palm is well known for its ability to accumulate large amounts of oil, approximately 63{\%} of the seed weight. Coconut oil varies significantly from other vegetable oils as it contains a high proportion of medium-chain fatty acids (MCFA; 85{\%}). The unique composition of coconut oil raises interest in understanding how the coconut palm produces oil of a high saturated MCFA content, and if such an oil profile could be replicated via biotechnology interventions. Although some gene discovery work has been performed there is still a significant gap in the knowledge associated with coconut's oil production pathways. In this study, a de novo transcriptome was assembled for developing coconut endosperm to identify genes involved in the synthesis of lipids, particularly triacylglycerol. Of particular interest were thioesterases, acyltransferases and oleosins because of their involvement in the processes of releasing fatty acids for assembly, esterification of fatty acids into glycerolipids and protecting oils from degradation, respectively. It is hypothesized that some of these genes may exhibit a strong substrate preference for MCFA and hence may assist the future development of vegetable oils with an enriched MCFA composition. In this study, we identified and confirmed functionality of five candidate genes from the gene families of interest. This study will benefit future work in areas of increasing vegetable oil production and the tailoring of oil fatty acid compositions.",
keywords = "De novo assembly, Nicotiana, Coconut, DGAT, Endosperm, FATA, Fatty acid, GPAT9, Medium chain, Oleosin, PDAT, Thioesterase, Transcriptome, Triacylglycerol",
author = "Reynolds, {Kyle B.} and Cullerne, {Darren P.} and {El Tahchy}, Anna and Vivien Rolland and Blanchard, {Christopher L.} and Wood, {Craig C.} and Singh, {Surinder P.} and Petrie, {James R.}",
year = "2019",
month = "5",
day = "1",
doi = "10.1093/pcp/pcy247",
language = "English",
volume = "60",
pages = "945--960",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "5",

}

Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm. / Reynolds, Kyle B.; Cullerne, Darren P.; El Tahchy, Anna; Rolland, Vivien; Blanchard, Christopher L.; Wood, Craig C.; Singh, Surinder P.; Petrie, James R.

In: Plant & cell physiology, Vol. 60, No. 5, 01.05.2019, p. 945-960.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm

AU - Reynolds, Kyle B.

AU - Cullerne, Darren P.

AU - El Tahchy, Anna

AU - Rolland, Vivien

AU - Blanchard, Christopher L.

AU - Wood, Craig C.

AU - Singh, Surinder P.

AU - Petrie, James R.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Cocos nucifera (coconut), a member of the Arecaceae family, is an economically important woody palm that is widely grown in tropical and subtropical regions. The coconut palm is well known for its ability to accumulate large amounts of oil, approximately 63% of the seed weight. Coconut oil varies significantly from other vegetable oils as it contains a high proportion of medium-chain fatty acids (MCFA; 85%). The unique composition of coconut oil raises interest in understanding how the coconut palm produces oil of a high saturated MCFA content, and if such an oil profile could be replicated via biotechnology interventions. Although some gene discovery work has been performed there is still a significant gap in the knowledge associated with coconut's oil production pathways. In this study, a de novo transcriptome was assembled for developing coconut endosperm to identify genes involved in the synthesis of lipids, particularly triacylglycerol. Of particular interest were thioesterases, acyltransferases and oleosins because of their involvement in the processes of releasing fatty acids for assembly, esterification of fatty acids into glycerolipids and protecting oils from degradation, respectively. It is hypothesized that some of these genes may exhibit a strong substrate preference for MCFA and hence may assist the future development of vegetable oils with an enriched MCFA composition. In this study, we identified and confirmed functionality of five candidate genes from the gene families of interest. This study will benefit future work in areas of increasing vegetable oil production and the tailoring of oil fatty acid compositions.

AB - Cocos nucifera (coconut), a member of the Arecaceae family, is an economically important woody palm that is widely grown in tropical and subtropical regions. The coconut palm is well known for its ability to accumulate large amounts of oil, approximately 63% of the seed weight. Coconut oil varies significantly from other vegetable oils as it contains a high proportion of medium-chain fatty acids (MCFA; 85%). The unique composition of coconut oil raises interest in understanding how the coconut palm produces oil of a high saturated MCFA content, and if such an oil profile could be replicated via biotechnology interventions. Although some gene discovery work has been performed there is still a significant gap in the knowledge associated with coconut's oil production pathways. In this study, a de novo transcriptome was assembled for developing coconut endosperm to identify genes involved in the synthesis of lipids, particularly triacylglycerol. Of particular interest were thioesterases, acyltransferases and oleosins because of their involvement in the processes of releasing fatty acids for assembly, esterification of fatty acids into glycerolipids and protecting oils from degradation, respectively. It is hypothesized that some of these genes may exhibit a strong substrate preference for MCFA and hence may assist the future development of vegetable oils with an enriched MCFA composition. In this study, we identified and confirmed functionality of five candidate genes from the gene families of interest. This study will benefit future work in areas of increasing vegetable oil production and the tailoring of oil fatty acid compositions.

KW - De novo assembly

KW - Nicotiana

KW - Coconut

KW - DGAT

KW - Endosperm

KW - FATA

KW - Fatty acid

KW - GPAT9

KW - Medium chain

KW - Oleosin

KW - PDAT

KW - Thioesterase

KW - Transcriptome

KW - Triacylglycerol

UR - http://www.scopus.com/inward/record.url?scp=85065563126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065563126&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcy247

DO - 10.1093/pcp/pcy247

M3 - Article

VL - 60

SP - 945

EP - 960

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 5

ER -