Identification of invasive grass species via DNA barcoding

Research output: Book chapter/Published conference paperConference paper

Abstract

Early intervention and mitigation of invasive grasses in Australia is often confounded by problems of species mis-identification. Field identifications can be erroneous through lack of taxonomic expertise or facilities to accurately distinguish invasive grasses from native varieties. Here we report the utility of DNA barcoding as a genetic method for assisting in the identification of high profile invasive grasses present in Eastern Australia. A total of 606 grass specimens, including four major invasive grass species [Nassella neesiana (Trin. & Rupr.) Barkworth (Chilean needle grass), Nassella trichotoma (Nees) Hack. ex Arechav. (serrated tussock), Eragrostis curvula (Schrad.) Nees (African love grass), and Hyparrhenia hirta (L.) Stapf (Coolatai grass)], and a variety of native species were sampled across a broad geographic area. Up to18 genetic markers (including 17 chloroplast markers and one nuclear marker) were screened for their efficiencies as potential DNA barcodes for identifications of weeds species. Preliminary results indicate that five genetic regions (ITS, matK, atpF, ndhK and petL) displayed some facility in separating particular species from others, but no single gene was suitable as a universal standalone DNA barcode region for accurate identification of all the surveyed species. Our future work will focus on these five genes to improve their PCR success rate across species, and to determine if multi-locus DNA barcodeprofiles can be used to distinguish all species in specific genera.
Original languageEnglish
Title of host publicationProceedings of the 18th NSW Weeds Conference
Subtitle of host publicationWeeds - The Future, Innovation and Adaptation
Pages68-81
Number of pages14
Publication statusPublished - 2015
Event18th NSW Weeds Conference - Multi-Function Centre, Cooma, Australia
Duration: 12 Oct 201515 Oct 2015
http://www.nswweedsoc.org.au/about_us/objectives/

Conference

Conference18th NSW Weeds Conference
Abbreviated titleWeeds - The Future, Innovation and Adaptation
CountryAustralia
CityCooma
Period12/10/1515/10/15
Internet address

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    Wang, A., Gopurenko, D., Wu, H., Stanton, R., & Lepschi, B. J. (2015). Identification of invasive grass species via DNA barcoding. In Proceedings of the 18th NSW Weeds Conference : Weeds - The Future, Innovation and Adaptation (pp. 68-81)