Identification of loci associated with Pleiochaeta setosa in white lupin

SML analysis identified two DArT marker loci (IPms-751082 and IPa-751380) which showed association with PRR resistance at a significance level of p <0.05. Both these markers, IPms-751082 and IPa-751380 exhibited significant differences in allele 1� frequency between two distribution tails of 0.49 and 0.43, respectively, as expected. DArT Toolbox was used to locate the neighbouring DArT markers close to IPms-751082 and IPa-751380 loci. Results showed that IPms-751082 marker is closely linked (16% humming distance) with IPms-532783 which was mapped on linkage group 1 (LG1) in the integrated map of P25758/Kiev Mutant population. No other neighbouring close markers for IPa-751380 were identified. Furthermore these markers could not be mapped to the new integrated genetic map of white lupin population derived from Kiev Mutant/P27174 (in this study) as they were monomorphic between parental lines of this mapping population. In order to map DArT markers on the white lupin genome, a subset of 96 F8 RIL derived from Kiev Mutant/P27174 were utilised. This RIL population has been previously used for mapping more than 300 genic and amplified fragment length polymorphism (AFLP) marker, and quantitative trait loci (QTL) associated with anthracnose resistance, flowering time and alkaloid content. In this study, the DArT markers were mapped for the first time in a RIL population derived from Kiev Mutant/P27174. A total of 136 DArT polymorphic markers were integrated in the new map along with previously mapped AFLPs and other genic markers.