Abstract
Bovine peptidoglycan recognition proteins (PGRPs) are conserved
innate immune pattern recognition molecules (PRMs).
Different PGRPs have been reported to recognize and digest
peptidoglycan (PGN) in both human and bovine populations.
Bovine PGRP1 (bPGRP1) is a unique secretory PGRP that
shows affinity for multiple pathogen associated molecular
patterns, including lipopolysaccharide, lipotechoic acid and
PGN. Bovine PGRP1 is the first PGRP reported to kill
microorganisms in vitro, and has been shown to play an
important role in the killing of phagocytosed bacteria by
neutrophils. The objective of this study was to identify single
nucleotide polymorphisms (SNP) in bPGRP1 and evaluate the
association of these SNP with two important inflammatory
diseases in dairy cattle, mastitis, and Johne’s disease (JD)
caused by Mycobacterium avium paratuberculosis (MAP). A
selective DNA pool was constructed based on the estimated
breeding values (EBV) for milk somatic cell score (SCS), and
gene segments were amplified in PCR reactions; these PCR
amplicons were sequenced to reveal SNPs in the bPGRP1
gene. A total of three SNP (c.102GC, c.480GA and
c.625CA) were identified that included one synonymous
SNP, one non-synonymous SNP, and one SNP in the 3?
untranslated region. All three SNP were genotyped for
individual samples included in two different DNA banks;
one made up of approximately 500 Holstein bulls with known
EBV for SCS, and the other made up of more than 500
Holstein cows tested for MAP infection. The statistical
analysis revealed significant differences in allelic frequencies
between MAP positive and negative cows for all the three SNP
(PB0.0008, PB0.0037, PB0.008). Analysis of the genotypes
also revealed statistically significant associations between
genotypes of all three SNP and MAP infection status (PB
0.002, PB0.0096, PB0.0189). Statistical analysis did not
reveal any significant associations with SCS EBV. These
results indicate a possible link between bPGRP1 SNP and
Map infection status and warrants further investigation.
innate immune pattern recognition molecules (PRMs).
Different PGRPs have been reported to recognize and digest
peptidoglycan (PGN) in both human and bovine populations.
Bovine PGRP1 (bPGRP1) is a unique secretory PGRP that
shows affinity for multiple pathogen associated molecular
patterns, including lipopolysaccharide, lipotechoic acid and
PGN. Bovine PGRP1 is the first PGRP reported to kill
microorganisms in vitro, and has been shown to play an
important role in the killing of phagocytosed bacteria by
neutrophils. The objective of this study was to identify single
nucleotide polymorphisms (SNP) in bPGRP1 and evaluate the
association of these SNP with two important inflammatory
diseases in dairy cattle, mastitis, and Johne’s disease (JD)
caused by Mycobacterium avium paratuberculosis (MAP). A
selective DNA pool was constructed based on the estimated
breeding values (EBV) for milk somatic cell score (SCS), and
gene segments were amplified in PCR reactions; these PCR
amplicons were sequenced to reveal SNPs in the bPGRP1
gene. A total of three SNP (c.102GC, c.480GA and
c.625CA) were identified that included one synonymous
SNP, one non-synonymous SNP, and one SNP in the 3?
untranslated region. All three SNP were genotyped for
individual samples included in two different DNA banks;
one made up of approximately 500 Holstein bulls with known
EBV for SCS, and the other made up of more than 500
Holstein cows tested for MAP infection. The statistical
analysis revealed significant differences in allelic frequencies
between MAP positive and negative cows for all the three SNP
(PB0.0008, PB0.0037, PB0.008). Analysis of the genotypes
also revealed statistically significant associations between
genotypes of all three SNP and MAP infection status (PB
0.002, PB0.0096, PB0.0189). Statistical analysis did not
reveal any significant associations with SCS EBV. These
results indicate a possible link between bPGRP1 SNP and
Map infection status and warrants further investigation.
| Original language | English |
|---|---|
| Pages | 157-158 |
| Number of pages | 2 |
| Publication status | Published - 2009 |
| Event | Canadian Society of Animal Science Annual Meeting - Montreal, Quebec, Canada Duration: 16 Mar 2008 → 18 Mar 2008 |
Conference
| Conference | Canadian Society of Animal Science Annual Meeting |
|---|---|
| Country/Territory | Canada |
| City | Montreal, Quebec |
| Period | 16/03/08 → 18/03/08 |