Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia: A morphological and molecular approach

Research output: Book chapter/Published conference paperConference paper

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Abstract

Camel melon (Citrullus lanatus (Thunb.)Matsum. & Nakai var. lanatus), colocynth (Citrulluscolocynthis (L.) Schrad.), and prickly paddy melon(Cucumis myriocarpus L.), are summer growinginvasive weeds found throughout Australia. Theyinfest both natural and agricultural ecosystems andare noxious weeds in some areas of Australia. Camelmelon and prickly paddy melon are annuals, whilecolocynth is a perennial. Camel melon and pricklymelon belong to different genera but as they sharesimilar morphology and life history they are oftenmisidentified at the vegetative stage. In this study, amolecular and morphological approach was used tofacilitate identification of these melon species. Formolecular taxonomic identification, two chloroplastgenes matK and ycf6'psbM intergenic spacer anda nuclear gene, G3pdh were used to identify theseinvasives. The sequences of G3pdh and ycf6'psbMidentified camel melon as C. lanatus var. citroides andthe colocynth as C. colocynthis, in direct contrast tothe current widely published nomenclature (as above).Australian prickly paddy melon sequences at matK revealedit to be Cucumis myriocarpus. For morphologicalcharacterisation, populations of each species fromselected Australian states were grown in a glass houseover a 4-month period in 2011. Each species exhibiteddistinct leaf lobation, branching of tendrils, floral, fruitand seed attributes, all of which are presented as usefulidentifying features. This study found that camelmelon, colocynth and prickly paddy melon possessedunique morphological characteristics. In addition, eachweed was identified to species level using multi locusDNA sequence analysis, demonstrating the utility ofthis approach for resolving nomenclatural errors andtaxonomic mis-identifications.
Original languageEnglish
Title of host publicationProceedings of the 18th Australasian Weeds Conference (2012)
Subtitle of host publicationDeveloping Solutions to Evolving Weed Problems
EditorsValerie Eldershaw
Place of PublicationMelbourne
PublisherWeed Society of Victoria
Pages73-77
Number of pages5
ISBN (Electronic)9780646586700
Publication statusPublished - 2012
Event18th Australasian Weeds Conference (2012) - The Sebel and Citigate Albert Park, Melbourne, Australia
Duration: 08 Oct 201211 Oct 2012
http://caws.org.au/awc_contents.php?yr=2012 (Conference proceedings)

Conference

Conference18th Australasian Weeds Conference (2012)
Abbreviated titleDeveloping Solutions to Evolving Weed Problems
CountryAustralia
CityMelbourne
Period08/10/1211/10/12
Internet address

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camels
melons
paddies
weeds
Citrullus lanatus var. citroides
noxious weeds
Citrullus lanatus
agroecosystems
intergenic DNA
vegetative growth
branching
glass
sequence analysis
life history
summer
seeds
leaves

Cite this

Shaik, R., Gopurenko, D., Burrows, G., Urwin, N., Hildebrand, S., Lepschi, B., & Weston, L. (2012). Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia: A morphological and molecular approach. In V. Eldershaw (Ed.), Proceedings of the 18th Australasian Weeds Conference (2012): Developing Solutions to Evolving Weed Problems (pp. 73-77). Melbourne: Weed Society of Victoria.
Shaik, Razia ; Gopurenko, David ; Burrows, Geoffrey ; Urwin, Nigel ; Hildebrand, Shane ; Lepschi, Brendan ; Weston, Leslie. / Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia : A morphological and molecular approach. Proceedings of the 18th Australasian Weeds Conference (2012): Developing Solutions to Evolving Weed Problems. editor / Valerie Eldershaw. Melbourne : Weed Society of Victoria, 2012. pp. 73-77
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title = "Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia: A morphological and molecular approach",
abstract = "Camel melon (Citrullus lanatus (Thunb.)Matsum. & Nakai var. lanatus), colocynth (Citrulluscolocynthis (L.) Schrad.), and prickly paddy melon(Cucumis myriocarpus L.), are summer growinginvasive weeds found throughout Australia. Theyinfest both natural and agricultural ecosystems andare noxious weeds in some areas of Australia. Camelmelon and prickly paddy melon are annuals, whilecolocynth is a perennial. Camel melon and pricklymelon belong to different genera but as they sharesimilar morphology and life history they are oftenmisidentified at the vegetative stage. In this study, amolecular and morphological approach was used tofacilitate identification of these melon species. Formolecular taxonomic identification, two chloroplastgenes matK and ycf6'psbM intergenic spacer anda nuclear gene, G3pdh were used to identify theseinvasives. The sequences of G3pdh and ycf6'psbMidentified camel melon as C. lanatus var. citroides andthe colocynth as C. colocynthis, in direct contrast tothe current widely published nomenclature (as above).Australian prickly paddy melon sequences at matK revealedit to be Cucumis myriocarpus. For morphologicalcharacterisation, populations of each species fromselected Australian states were grown in a glass houseover a 4-month period in 2011. Each species exhibiteddistinct leaf lobation, branching of tendrils, floral, fruitand seed attributes, all of which are presented as usefulidentifying features. This study found that camelmelon, colocynth and prickly paddy melon possessedunique morphological characteristics. In addition, eachweed was identified to species level using multi locusDNA sequence analysis, demonstrating the utility ofthis approach for resolving nomenclatural errors andtaxonomic mis-identifications.",
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author = "Razia Shaik and David Gopurenko and Geoffrey Burrows and Nigel Urwin and Shane Hildebrand and Brendan Lepschi and Leslie Weston",
note = "Imported on 03 May 2017 - DigiTool details were: publisher = Melbourne: Weed Society of Victoria, 2012. editor/s (773b) = Valerie Eldershaw; Event dates (773o) = 8-11 October 2012; Parent title (773t) = Australasian Weeds Conference (AWC).",
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Shaik, R, Gopurenko, D, Burrows, G, Urwin, N, Hildebrand, S, Lepschi, B & Weston, L 2012, Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia: A morphological and molecular approach. in V Eldershaw (ed.), Proceedings of the 18th Australasian Weeds Conference (2012): Developing Solutions to Evolving Weed Problems. Weed Society of Victoria, Melbourne, pp. 73-77, 18th Australasian Weeds Conference (2012), Melbourne, Australia, 08/10/12.

Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia : A morphological and molecular approach. / Shaik, Razia; Gopurenko, David; Burrows, Geoffrey; Urwin, Nigel; Hildebrand, Shane; Lepschi, Brendan; Weston, Leslie.

Proceedings of the 18th Australasian Weeds Conference (2012): Developing Solutions to Evolving Weed Problems. ed. / Valerie Eldershaw. Melbourne : Weed Society of Victoria, 2012. p. 73-77.

Research output: Book chapter/Published conference paperConference paper

TY - GEN

T1 - Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia

T2 - A morphological and molecular approach

AU - Shaik, Razia

AU - Gopurenko, David

AU - Burrows, Geoffrey

AU - Urwin, Nigel

AU - Hildebrand, Shane

AU - Lepschi, Brendan

AU - Weston, Leslie

N1 - Imported on 03 May 2017 - DigiTool details were: publisher = Melbourne: Weed Society of Victoria, 2012. editor/s (773b) = Valerie Eldershaw; Event dates (773o) = 8-11 October 2012; Parent title (773t) = Australasian Weeds Conference (AWC).

PY - 2012

Y1 - 2012

N2 - Camel melon (Citrullus lanatus (Thunb.)Matsum. & Nakai var. lanatus), colocynth (Citrulluscolocynthis (L.) Schrad.), and prickly paddy melon(Cucumis myriocarpus L.), are summer growinginvasive weeds found throughout Australia. Theyinfest both natural and agricultural ecosystems andare noxious weeds in some areas of Australia. Camelmelon and prickly paddy melon are annuals, whilecolocynth is a perennial. Camel melon and pricklymelon belong to different genera but as they sharesimilar morphology and life history they are oftenmisidentified at the vegetative stage. In this study, amolecular and morphological approach was used tofacilitate identification of these melon species. Formolecular taxonomic identification, two chloroplastgenes matK and ycf6'psbM intergenic spacer anda nuclear gene, G3pdh were used to identify theseinvasives. The sequences of G3pdh and ycf6'psbMidentified camel melon as C. lanatus var. citroides andthe colocynth as C. colocynthis, in direct contrast tothe current widely published nomenclature (as above).Australian prickly paddy melon sequences at matK revealedit to be Cucumis myriocarpus. For morphologicalcharacterisation, populations of each species fromselected Australian states were grown in a glass houseover a 4-month period in 2011. Each species exhibiteddistinct leaf lobation, branching of tendrils, floral, fruitand seed attributes, all of which are presented as usefulidentifying features. This study found that camelmelon, colocynth and prickly paddy melon possessedunique morphological characteristics. In addition, eachweed was identified to species level using multi locusDNA sequence analysis, demonstrating the utility ofthis approach for resolving nomenclatural errors andtaxonomic mis-identifications.

AB - Camel melon (Citrullus lanatus (Thunb.)Matsum. & Nakai var. lanatus), colocynth (Citrulluscolocynthis (L.) Schrad.), and prickly paddy melon(Cucumis myriocarpus L.), are summer growinginvasive weeds found throughout Australia. Theyinfest both natural and agricultural ecosystems andare noxious weeds in some areas of Australia. Camelmelon and prickly paddy melon are annuals, whilecolocynth is a perennial. Camel melon and pricklymelon belong to different genera but as they sharesimilar morphology and life history they are oftenmisidentified at the vegetative stage. In this study, amolecular and morphological approach was used tofacilitate identification of these melon species. Formolecular taxonomic identification, two chloroplastgenes matK and ycf6'psbM intergenic spacer anda nuclear gene, G3pdh were used to identify theseinvasives. The sequences of G3pdh and ycf6'psbMidentified camel melon as C. lanatus var. citroides andthe colocynth as C. colocynthis, in direct contrast tothe current widely published nomenclature (as above).Australian prickly paddy melon sequences at matK revealedit to be Cucumis myriocarpus. For morphologicalcharacterisation, populations of each species fromselected Australian states were grown in a glass houseover a 4-month period in 2011. Each species exhibiteddistinct leaf lobation, branching of tendrils, floral, fruitand seed attributes, all of which are presented as usefulidentifying features. This study found that camelmelon, colocynth and prickly paddy melon possessedunique morphological characteristics. In addition, eachweed was identified to species level using multi locusDNA sequence analysis, demonstrating the utility ofthis approach for resolving nomenclatural errors andtaxonomic mis-identifications.

KW - Open access version available

KW - Keywords Cucurbitaceous invasive weeds

M3 - Conference paper

SP - 73

EP - 77

BT - Proceedings of the 18th Australasian Weeds Conference (2012)

A2 - Eldershaw, Valerie

PB - Weed Society of Victoria

CY - Melbourne

ER -

Shaik R, Gopurenko D, Burrows G, Urwin N, Hildebrand S, Lepschi B et al. Identification of the invasive weeds, camel melon, prickly paddy melon and colocynth in Australia: A morphological and molecular approach. In Eldershaw V, editor, Proceedings of the 18th Australasian Weeds Conference (2012): Developing Solutions to Evolving Weed Problems. Melbourne: Weed Society of Victoria. 2012. p. 73-77