Implications of bacterial contaminated seed lots and endophytic colonization by Pseudomonas fuscovaginae on rice establishment

Dante Adorada, Benjamin Stodart, I.B. Pangga, Gavin Ash

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The economic impact of seedborne bacterial diseases on rice production provides a major motivation for research on seed health. This paper reports on the endophytic growth of a rifampicin-marked strain of the seedborne rice pathogen Pseudomonas fuscovaginae. The bacterium was found in most tested seeds indicating that, even without visible discolouration, seed transmission is possible. Crushed discoloured seeds contained more bacterial cells than did non-crushed discoloured seeds. These bacteria were released during seed soaking, contaminating clean seed and lowering seed germination. Cells of a rifampicin-resistant strain of P. fuscovaginae, which had been inoculated onto rice seeds, were subsequently recovered from different growth stages and from different rice tissues, thereby indicating endophytic colonization. These results have implications for seedling establishment, as symptomless seeds do not assure disease-free seeds, and the presence of seedborne bacteria results in poor germination and poor seedling establishment. Elimination of seedborne bacteria by soaking in sodium hypochlorite can increase seed germination. This could be used in developing control strategies, and, if practised regularly, reduce entry of seedborne disease-causing organisms into crops, resulting in lower disease pressure.
Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalPlant Pathology
Volume64
Issue number1
Early online dateJun 2014
DOIs
Publication statusPublished - Feb 2015

Fingerprint

Pseudomonas fuscovaginae
Pseudomonas
Seeds
rice
seeds
seed-borne bacteria
Germination
rifampicin
Bacteria
soaking
Rifampin
seed germination
Seedlings
seed-borne diseases
Oryza
seedlings
sodium hypochlorite
bacteria
Sodium Hypochlorite
economic impact

Cite this

@article{48a1e7b381e848358037198a16108c95,
title = "Implications of bacterial contaminated seed lots and endophytic colonization by Pseudomonas fuscovaginae on rice establishment",
abstract = "The economic impact of seedborne bacterial diseases on rice production provides a major motivation for research on seed health. This paper reports on the endophytic growth of a rifampicin-marked strain of the seedborne rice pathogen Pseudomonas fuscovaginae. The bacterium was found in most tested seeds indicating that, even without visible discolouration, seed transmission is possible. Crushed discoloured seeds contained more bacterial cells than did non-crushed discoloured seeds. These bacteria were released during seed soaking, contaminating clean seed and lowering seed germination. Cells of a rifampicin-resistant strain of P. fuscovaginae, which had been inoculated onto rice seeds, were subsequently recovered from different growth stages and from different rice tissues, thereby indicating endophytic colonization. These results have implications for seedling establishment, as symptomless seeds do not assure disease-free seeds, and the presence of seedborne bacteria results in poor germination and poor seedling establishment. Elimination of seedborne bacteria by soaking in sodium hypochlorite can increase seed germination. This could be used in developing control strategies, and, if practised regularly, reduce entry of seedborne disease-causing organisms into crops, resulting in lower disease pressure.",
keywords = "discolouration, endophytes, germination, refampicin-resistant, seed lot infection, seedborne",
author = "Dante Adorada and Benjamin Stodart and I.B. Pangga and Gavin Ash",
note = "Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Plant Pathology. ISSNs: 1365-3059;",
year = "2015",
month = "2",
doi = "10.1111/ppa.12243",
language = "English",
volume = "64",
pages = "43--50",
journal = "Plant Pathology",
issn = "0032-0862",
publisher = "Wiley-Blackwell",
number = "1",

}

Implications of bacterial contaminated seed lots and endophytic colonization by Pseudomonas fuscovaginae on rice establishment. / Adorada, Dante; Stodart, Benjamin; Pangga, I.B.; Ash, Gavin.

In: Plant Pathology, Vol. 64, No. 1, 02.2015, p. 43-50.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Implications of bacterial contaminated seed lots and endophytic colonization by Pseudomonas fuscovaginae on rice establishment

AU - Adorada, Dante

AU - Stodart, Benjamin

AU - Pangga, I.B.

AU - Ash, Gavin

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Plant Pathology. ISSNs: 1365-3059;

PY - 2015/2

Y1 - 2015/2

N2 - The economic impact of seedborne bacterial diseases on rice production provides a major motivation for research on seed health. This paper reports on the endophytic growth of a rifampicin-marked strain of the seedborne rice pathogen Pseudomonas fuscovaginae. The bacterium was found in most tested seeds indicating that, even without visible discolouration, seed transmission is possible. Crushed discoloured seeds contained more bacterial cells than did non-crushed discoloured seeds. These bacteria were released during seed soaking, contaminating clean seed and lowering seed germination. Cells of a rifampicin-resistant strain of P. fuscovaginae, which had been inoculated onto rice seeds, were subsequently recovered from different growth stages and from different rice tissues, thereby indicating endophytic colonization. These results have implications for seedling establishment, as symptomless seeds do not assure disease-free seeds, and the presence of seedborne bacteria results in poor germination and poor seedling establishment. Elimination of seedborne bacteria by soaking in sodium hypochlorite can increase seed germination. This could be used in developing control strategies, and, if practised regularly, reduce entry of seedborne disease-causing organisms into crops, resulting in lower disease pressure.

AB - The economic impact of seedborne bacterial diseases on rice production provides a major motivation for research on seed health. This paper reports on the endophytic growth of a rifampicin-marked strain of the seedborne rice pathogen Pseudomonas fuscovaginae. The bacterium was found in most tested seeds indicating that, even without visible discolouration, seed transmission is possible. Crushed discoloured seeds contained more bacterial cells than did non-crushed discoloured seeds. These bacteria were released during seed soaking, contaminating clean seed and lowering seed germination. Cells of a rifampicin-resistant strain of P. fuscovaginae, which had been inoculated onto rice seeds, were subsequently recovered from different growth stages and from different rice tissues, thereby indicating endophytic colonization. These results have implications for seedling establishment, as symptomless seeds do not assure disease-free seeds, and the presence of seedborne bacteria results in poor germination and poor seedling establishment. Elimination of seedborne bacteria by soaking in sodium hypochlorite can increase seed germination. This could be used in developing control strategies, and, if practised regularly, reduce entry of seedborne disease-causing organisms into crops, resulting in lower disease pressure.

KW - discolouration

KW - endophytes

KW - germination

KW - refampicin-resistant

KW - seed lot infection

KW - seedborne

U2 - 10.1111/ppa.12243

DO - 10.1111/ppa.12243

M3 - Article

VL - 64

SP - 43

EP - 50

JO - Plant Pathology

JF - Plant Pathology

SN - 0032-0862

IS - 1

ER -