TY - JOUR
T1 - Improvement of left ventricular remodelling by inhibition of NF-κB in a rat model of myocardial infarction
AU - Jin, Jin-lan
AU - Lv, Rong-gui
AU - Guo, Jian
AU - Liu, Xi-hong
AU - Liang, Yan-wen
AU - Wei, Jian-rui
AU - Wang, Lexin
N1 - Includes bibliographical references.
PY - 2016/10
Y1 - 2016/10
N2 - Background: To investigate the effects of inhibition of NF-?B activation on left ventricular (LV) remodelling in a rat model of myocardial infarction (MI). Methods: The acute MI model was established by ligation of left anterior descending coronary artery. Pyrrolidine dithiocarbamate (PDTC) (20. mg/kg, Qd) was administered intraperitoneally to inhibit NF-?B activation. Eight weeks later, the cardiac structure and LV ejection fraction were assessed with echocardiography. The rat body, heart, and LV weights were measured to calculate LV mass indices. Activation of NF-?B in non-infarcted myocardium was detected by a TransAM NF-?B p65 Transcription Factor Assay Kit. Cardiac collagen volume fraction was evaluated by Masson staining. Results: Eight weeks after the MI model was established, the LV posterior wall thickness in PDTC and MI group was 1.75. ±. 0.07. mm and 1.85. ±. 0.07. mm respectively (p <. 0.05). The LV mass index in the PDTC group (2.53. ±. 0.09) was lower than in the MI group (2.65. ±. 0.08, p <. 0.05). The LVEF in the PDTC group (63.89%. ±. 4.21%) was higher than in the MI group (42.73%. ±. 8.94%, p. <. 0.05). The interstitial collagen deposition in the non-infarcted myocardium in the PDTC group was less than in the MI group (7.25%. ±. 1.88% vs. 10.09%. ±. 2.19%, p <. 0.05). Conclusion: Inhibition of activation of NF-?B may result in improvement of myocardial remodelling after myocardial infarction, which is possibly attributable to reduced collagen deposition in non-infarcted areas.
AB - Background: To investigate the effects of inhibition of NF-?B activation on left ventricular (LV) remodelling in a rat model of myocardial infarction (MI). Methods: The acute MI model was established by ligation of left anterior descending coronary artery. Pyrrolidine dithiocarbamate (PDTC) (20. mg/kg, Qd) was administered intraperitoneally to inhibit NF-?B activation. Eight weeks later, the cardiac structure and LV ejection fraction were assessed with echocardiography. The rat body, heart, and LV weights were measured to calculate LV mass indices. Activation of NF-?B in non-infarcted myocardium was detected by a TransAM NF-?B p65 Transcription Factor Assay Kit. Cardiac collagen volume fraction was evaluated by Masson staining. Results: Eight weeks after the MI model was established, the LV posterior wall thickness in PDTC and MI group was 1.75. ±. 0.07. mm and 1.85. ±. 0.07. mm respectively (p <. 0.05). The LV mass index in the PDTC group (2.53. ±. 0.09) was lower than in the MI group (2.65. ±. 0.08, p <. 0.05). The LVEF in the PDTC group (63.89%. ±. 4.21%) was higher than in the MI group (42.73%. ±. 8.94%, p. <. 0.05). The interstitial collagen deposition in the non-infarcted myocardium in the PDTC group was less than in the MI group (7.25%. ±. 1.88% vs. 10.09%. ±. 2.19%, p <. 0.05). Conclusion: Inhibition of activation of NF-?B may result in improvement of myocardial remodelling after myocardial infarction, which is possibly attributable to reduced collagen deposition in non-infarcted areas.
KW - LV ejection fraction
KW - LV mass index
KW - Myocardial infarction
KW - NF-kB
KW - Ventricular remodelling
U2 - 10.1016/j.hlc.2015.11.005
DO - 10.1016/j.hlc.2015.11.005
M3 - Article
C2 - 27118230
SN - 1443-9506
VL - 25
SP - 1007
EP - 1012
JO - Heart Lung and Circulation
JF - Heart Lung and Circulation
IS - 10
ER -