In vitro investigations of the potential health benefits of Australian-grown faba beans (Vicia faba L.): chemopreventative capacity and inhibitory effects on the angiotensin-converting enzyme, a-glucosidase and lipase

Siem Siah, I. Konczak, Samson Agboola, J.A. Wood, Christopher Blanchard

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)
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Abstract

The functional properties, including antioxidant and chemopreventative capacities as well as the inhibitory effects on angiotensin-convertingenzyme (ACE), a-glucosidase and pancreatic lipase, of three Australian-grown faba bean genotypes (Nura, Rossa and TF(Ic*As)*483/13)were investigated using an array of in vitro assays. Chromatograms of on-line post column derivatisation assay coupled with HPLCrevealed the existence of active phenolics (hump) in the coloured genotypes, which was lacking in the white-coloured breeding line,TF(Ic*As)*483/13. Roasting reduced the phenolic content, and diminished antioxidant activity by 10'40% as measured by the reagentbasedassays (diphenylpicrylhydrazyl, 2,20-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) and oxygen radical absorbance capacity) inall genotypes. Cell culture-based antioxidant activity assay (cellular antioxidant activity) showed an increase of activity in the colouredgenotypes after roasting. Faba bean extracts demonstrated cellular protection ability against H2O2-induced DNA damage (assessedusing RAW264.7 cells), and inhibited the proliferation of all human cancer cell lines (BL13, AGS, Hep G2 and HT-29) evaluated. However,the effect of faba bean extracts on the non-transformed human cells (CCD-18Co) was negligible. Flow cytometric analyses showed thatfaba bean extracts successfully induced apoptosis of HL-60 (acute promyelocytic leukaemia) cells. The faba bean extracts also exhibitedACE, a-glucosidase and pancreatic lipase inhibitory activities. Overall, extracts from Nura (buff-coloured) and Rossa (red-coloured) werecomparable, while TF(Ic*As)*483/13 (white-coloured) contained the lowest phenolic content and exhibited the least antioxidant andenzyme inhibition activities. These results are important to promote the utilisation of faba beans in human diets for various health benefits
Original languageEnglish
Pages (from-to)123-134
Number of pages12
JournalBritish Journal of Nutrition
Volume108
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Aug 2012

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