Infection process of Plectosporium alismatis on host and non-host species in the Alismataceae

Wayne Pitt, Eric J. Cother, Norma J. Cother, Gavin Ash

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In Australia, the endemic fungus Plectosporium alismatis (syn. Rhynchosporium alismatis) has potential use as a mycoherbicide for several species in the Alismataceae, a family of aquatic and semi-aquatic marsh herbs, which are considered to be important weeds in rice crops. Of five species identified in south-eastern Australia where rice is grown, two species, Sagittaria graminea and Sagittaria montevidensis are resistant (non-hosts), and no records of P. alismatis on these species have been reported. To better understand the interactions that lead to resistance in these pathosystems, the infection process of the fungus was studied on these species and also on the host Alisma plantago-aquatica, using light, fluorescent and scanning electron microscopy. On all three species both conidial germination and appressorium formation commenced within 6 h of inoculation with greater than 50% of conidia elongating to form germ tube structures and associated appressoria 12'18 h post inoculation. Germ tube elongation and appressorium formation occurred randomly over the leaf surface. Direct host penetration was facilitated by the production of penetration hyphae that emerged from beneath appressoria. Penetration sites were clearly identified by the presence of spherical holes 0.25'0.5 'm in diam, and were frequently accompanied by resistance reactions in non-host species. Visible symptoms of disease occurred 4'6 d after inoculation of susceptible (host) species.
Original languageEnglish
Pages (from-to)837-845
Number of pages9
JournalFungal Biology
Volume108
Issue number7
DOIs
Publication statusPublished - 2004

Fingerprint

Alismataceae
Sagittaria
appressoria
Alisma
South Australia
Fungal Spores
Hyphae
Mycoses
Wetlands
germ tube
Germination
Infection
infection
Electron Scanning Microscopy
Alisma plantago-aquatica
Rhynchosporium
Fungi
mycoherbicides
inoculation
Light

Cite this

Pitt, Wayne ; Cother, Eric J. ; Cother, Norma J. ; Ash, Gavin. / Infection process of Plectosporium alismatis on host and non-host species in the Alismataceae. In: Fungal Biology. 2004 ; Vol. 108, No. 7. pp. 837-845.
@article{88c16484faa54505bc00751d9b52e33c,
title = "Infection process of Plectosporium alismatis on host and non-host species in the Alismataceae",
abstract = "In Australia, the endemic fungus Plectosporium alismatis (syn. Rhynchosporium alismatis) has potential use as a mycoherbicide for several species in the Alismataceae, a family of aquatic and semi-aquatic marsh herbs, which are considered to be important weeds in rice crops. Of five species identified in south-eastern Australia where rice is grown, two species, Sagittaria graminea and Sagittaria montevidensis are resistant (non-hosts), and no records of P. alismatis on these species have been reported. To better understand the interactions that lead to resistance in these pathosystems, the infection process of the fungus was studied on these species and also on the host Alisma plantago-aquatica, using light, fluorescent and scanning electron microscopy. On all three species both conidial germination and appressorium formation commenced within 6 h of inoculation with greater than 50{\%} of conidia elongating to form germ tube structures and associated appressoria 12'18 h post inoculation. Germ tube elongation and appressorium formation occurred randomly over the leaf surface. Direct host penetration was facilitated by the production of penetration hyphae that emerged from beneath appressoria. Penetration sites were clearly identified by the presence of spherical holes 0.25'0.5 'm in diam, and were frequently accompanied by resistance reactions in non-host species. Visible symptoms of disease occurred 4'6 d after inoculation of susceptible (host) species.",
author = "Wayne Pitt and Cother, {Eric J.} and Cother, {Norma J.} and Gavin Ash",
note = "Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Mycological Research. ISSNs: 0953-7562;",
year = "2004",
doi = "10.1017/S0953756204000024",
language = "English",
volume = "108",
pages = "837--845",
journal = "Mycological Research",
issn = "0953-7562",
publisher = "Elsevier",
number = "7",

}

Infection process of Plectosporium alismatis on host and non-host species in the Alismataceae. / Pitt, Wayne; Cother, Eric J.; Cother, Norma J.; Ash, Gavin.

In: Fungal Biology, Vol. 108, No. 7, 2004, p. 837-845.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Infection process of Plectosporium alismatis on host and non-host species in the Alismataceae

AU - Pitt, Wayne

AU - Cother, Eric J.

AU - Cother, Norma J.

AU - Ash, Gavin

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Mycological Research. ISSNs: 0953-7562;

PY - 2004

Y1 - 2004

N2 - In Australia, the endemic fungus Plectosporium alismatis (syn. Rhynchosporium alismatis) has potential use as a mycoherbicide for several species in the Alismataceae, a family of aquatic and semi-aquatic marsh herbs, which are considered to be important weeds in rice crops. Of five species identified in south-eastern Australia where rice is grown, two species, Sagittaria graminea and Sagittaria montevidensis are resistant (non-hosts), and no records of P. alismatis on these species have been reported. To better understand the interactions that lead to resistance in these pathosystems, the infection process of the fungus was studied on these species and also on the host Alisma plantago-aquatica, using light, fluorescent and scanning electron microscopy. On all three species both conidial germination and appressorium formation commenced within 6 h of inoculation with greater than 50% of conidia elongating to form germ tube structures and associated appressoria 12'18 h post inoculation. Germ tube elongation and appressorium formation occurred randomly over the leaf surface. Direct host penetration was facilitated by the production of penetration hyphae that emerged from beneath appressoria. Penetration sites were clearly identified by the presence of spherical holes 0.25'0.5 'm in diam, and were frequently accompanied by resistance reactions in non-host species. Visible symptoms of disease occurred 4'6 d after inoculation of susceptible (host) species.

AB - In Australia, the endemic fungus Plectosporium alismatis (syn. Rhynchosporium alismatis) has potential use as a mycoherbicide for several species in the Alismataceae, a family of aquatic and semi-aquatic marsh herbs, which are considered to be important weeds in rice crops. Of five species identified in south-eastern Australia where rice is grown, two species, Sagittaria graminea and Sagittaria montevidensis are resistant (non-hosts), and no records of P. alismatis on these species have been reported. To better understand the interactions that lead to resistance in these pathosystems, the infection process of the fungus was studied on these species and also on the host Alisma plantago-aquatica, using light, fluorescent and scanning electron microscopy. On all three species both conidial germination and appressorium formation commenced within 6 h of inoculation with greater than 50% of conidia elongating to form germ tube structures and associated appressoria 12'18 h post inoculation. Germ tube elongation and appressorium formation occurred randomly over the leaf surface. Direct host penetration was facilitated by the production of penetration hyphae that emerged from beneath appressoria. Penetration sites were clearly identified by the presence of spherical holes 0.25'0.5 'm in diam, and were frequently accompanied by resistance reactions in non-host species. Visible symptoms of disease occurred 4'6 d after inoculation of susceptible (host) species.

U2 - 10.1017/S0953756204000024

DO - 10.1017/S0953756204000024

M3 - Article

VL - 108

SP - 837

EP - 845

JO - Mycological Research

JF - Mycological Research

SN - 0953-7562

IS - 7

ER -