Influence of in situ progressive N-terminal is still controversial truncation of glycogen branching enzyme in Escherichia coli DH5α on glycogen structure, accumulation, and bacterial viability

Liang Wang, Ahmed Regina, Vito M. Butardo, Behjat Kosar-Hashemi, Oscar Larroque, Charlene M. Kahler, Michael J. Wise

Research output: Contribution to journalArticle

9 Citations (Scopus)
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Abstract

Glycogen average chain length (ACL) has been linked with bacterial durability, but this was on the basis of observations across different species. We therefore wished to investigate the relationship between bacterial durability and glycogen ACL by varying glycogen average chain length in a single species. It has been shown that progressive shortening of the N-terminus of glycogen branching enzyme (GBE) leads to a lengthening of oligosaccharide inter-α-1,6-glycosidic chain lengths, so we sought to harness this to create a set of Escherichia coli DH5α strains with a range of glycogen average chain lengths, and assess these strains for durability related attributes, such as starvation, cold and desiccation stress resistance, and biofilm formation. 
Original languageEnglish
Article number421
Pages (from-to)1-14
JournalBMC Microbiology
Volume15
Issue number1
DOIs
Publication statusPublished - 07 May 2015

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