Integration of a highly ordered gold nanowires array with glucose oxidase for ultra-sensitive glucose detection

Jiewu Cui, Samuel B. Adeloju, Yucheng Wu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A highly sensitive amperometric nanobiosensor has been developed by integration of glucose oxidase (GOx) with a gold nanowires array (AuNWA) by cross-linking with a mixture of glutaraldehyde (GLA) and bovine serum albumin (BSA). An initial investigation of the morphology of the synthesized AuNWA by field emission scanning electron microscopy (FESEM) and field emission transmission electron microscopy (FETEM) revealed that the nanowires array was highly ordered with rough surface, and the electrochemical features of the AuNWA with/without modification were also investigated. The integrated AuNWA-BSA-GLA-GOx nanobiosensor with Nafion membrane gave a very high sensitivity of 298.2μAcm-2mM-1 for amperometric detection of glucose, while also achieving a low detection limit of 0.1μM, and a wide linear range of 5-6000μM. Furthermore, the nanobiosensor exhibited excellent anti-interference ability towards uric acid (UA) and ascorbic acid (AA) with the aid of Nafion membrane, and the results obtained for the analysis of human blood serum indicated that the device is capable of glucose detection in real samples.

Original languageEnglish
Pages (from-to)134-140
Number of pages7
JournalAnalytica Chimica Acta
Volume809
Early online date19 Nov 2013
DOIs
Publication statusPublished - 27 Jan 2014

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Nanowires
Glucose Oxidase
Glutaral
Bovine Serum Albumin
Gold
Field emission
glucose
gold
Membranes
Glucose
serum
Uric Acid
Transmission Electron Microscopy
Electron Scanning Microscopy
Ascorbic Acid
Limit of Detection
Blood
membrane
Transmission electron microscopy
Equipment and Supplies

Cite this

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abstract = "A highly sensitive amperometric nanobiosensor has been developed by integration of glucose oxidase (GOx) with a gold nanowires array (AuNWA) by cross-linking with a mixture of glutaraldehyde (GLA) and bovine serum albumin (BSA). An initial investigation of the morphology of the synthesized AuNWA by field emission scanning electron microscopy (FESEM) and field emission transmission electron microscopy (FETEM) revealed that the nanowires array was highly ordered with rough surface, and the electrochemical features of the AuNWA with/without modification were also investigated. The integrated AuNWA-BSA-GLA-GOx nanobiosensor with Nafion membrane gave a very high sensitivity of 298.2μAcm-2mM-1 for amperometric detection of glucose, while also achieving a low detection limit of 0.1μM, and a wide linear range of 5-6000μM. Furthermore, the nanobiosensor exhibited excellent anti-interference ability towards uric acid (UA) and ascorbic acid (AA) with the aid of Nafion membrane, and the results obtained for the analysis of human blood serum indicated that the device is capable of glucose detection in real samples.",
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Integration of a highly ordered gold nanowires array with glucose oxidase for ultra-sensitive glucose detection. / Cui, Jiewu; Adeloju, Samuel B.; Wu, Yucheng.

In: Analytica Chimica Acta, Vol. 809, 27.01.2014, p. 134-140.

Research output: Contribution to journalArticle

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