Investigation of Australian olive mill waste for recovery of biophenols

Hassan Obied, Malcolm Allen, Danny Bedgood, Paul Prenzler, Kevin Robards

Research output: Contribution to journalArticlepeer-review

154 Citations (Scopus)


Olive mill waste is a potential source for the recovery of phytochemicals with a wide array of biological activities. Phytochemical screening of hexane, methanol, and water extracts revealed a diversity of compounds, perhaps overlooked in previous studies through intensive cleanup procedures. Methanol and water extracts contained large amounts of biophenols, and further testing of polar extraction solvents, including ethyl acetate, ethanol, propanol, acetone, acetonitrile, and water/methanol mixtures, highlighted the latter as the solvent of choice for extraction of the widest array of phenolic compounds. Stabilization of the resulting extract was best achieved by addition of 2% (w/w) sodium metabisulfite. Quantitative data are reported for nine biophenols extracted using 60% (v/v) methanol in water with 2% (w/w) sodium metabisulfite. Six compounds had recoveries of greater than 1 g/kg of freeze-dried waste: hydroxytyrosol glucoside, hydroxytyrosol, tyrosol, verbascoside, and a derivative of oleuropein.
Original languageEnglish
Pages (from-to)9911-9920
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Issue number26
Publication statusPublished - 2005


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