Investigation of microbes and surface carbohydrates using atomic force microscopy

Daniel Sijmons, Simon Collett, Lachlan Coff, Joy H. Kim, Emily Atwell, Anna K. Walduck, Aaron Elbourne, Paul A. Ramsland

Research output: Book chapter/Published conference paperChapter (peer-reviewed)peer-review

Abstract

Atomic force microscopy (AFM) is a useful tool in the imaging and investigation of surfaces and surface interactions. The use of the technique in microbiology has grown over the years, with publications using AFM to investigate various properties of microbes such as surface structures, membrane arrangements and even visualisation of individual proteins and cell division. AFM has also shown utility when it comes to measuring biological interactions, in particular receptor–ligand interactions, where the AFM is able to measure the strength of individual interactions and map the location of interactions to the surface location on the cell. Combining the use of AFM with binding assays and immunoassays provides the ability to profile and analyse the surface of microbes such as bacteria and viruses. Furthermore, utilising carbohydrate binding monoclonal antibodies and lectins provides the opportunity to better understand the glycosylation patterns of these cells and particles giving unique insights into how microbes react with the environment and binding agents. This chapter discusses the methods used in investigating microbes and the carbohydrates decorating their surfaces with AFM and immunoassays.

Original languageEnglish
Title of host publicationMicrobes at Bio/Nano Interfaces
EditorsPaul A. Ramsland, Paul A. Ramsland, Aaron Elbourne, Volker Gurtler
PublisherAcademic Press Inc.
Pages99-123
Number of pages25
ISBN (Print)9780443191190
DOIs
Publication statusPublished - Jan 2024

Publication series

NameMethods in Microbiology
Volume54
ISSN (Print)0580-9517

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